SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N) (His tag)

Produktnummer ABIN6952454

Produktdetails

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Protein-Typ: Recombinant

Spezies: SARS Coronavirus-2 (SARS-CoV-2)

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses SARS-CoV-2 Nucleocapsid Protein ist gelabelt mit His tag.

Sequenz: AA 1-419

Produktmerkmale: 2019-nCoV (COVID-19) Nucleocapsid protein, His Tag (NUN-C5227) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # YP_009724397.2). This protein carries a polyhistidine tag at the C-terminus.

Reinheit: >95 % as determined by SDS-PAGE.

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Applikationshinweise: The protein has a calculated MW of 47.3 kDa. The protein migrates as 60-65 kDa under reducing (R) condition due to glycosylation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: For long term storage, the product should be stored at lyophilized state at -20°C or lower. This product is stable aftr storage at: -20°C to -70°C for 12 months in lyophilized state, -70°C for 3 months under sterile conditions after reconstitution.

Referenzen

Sasisekharan, Pentakota, Jayaraman, Tharakaraman, Wogan, Narayanasami: "Orthogonal immunoassays for IgG antibodies to SARS-CoV-2 antigens reveal that immune response lasts beyond 4 mo post illness onset." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 118, Issue 5, (2021) (PubMed).

Supekar, Shajahan, Gleinich, Rouhani, Heiss, Chapla, Moremen, Azadi: "Variable post-translational modifications of SARS-CoV-2 nucleocapsid protein." in: Glycobiology, (2021) (PubMed).

Qian, Lei, Patel, Lee, Monaghan-Nichols, Xin, Qiu, Fu: "Direct activation of endothelial cells by SARS-CoV-2 nucleocapsid protein is blocked by Simvastatin." in: bioRxiv : the preprint server for biology, (2021) (PubMed).

Longworth, Dittmar: "An antigen microarray protocol for COVID-19 serological analysis." in: STAR protocols, Vol. 2, Issue 3, pp. 100815, (2021) (PubMed).

Rudberg, Havervall, Månberg, Jernbom Falk, Aguilera, Ng, Gabrielsson, Salomonsson, Hanke, Murrell, McInerney, Olofsson, Andersson, Hellström, Bayati, Bergström, Pin, Sjöberg, Tegel, Hedhammar et al.: "SARS-CoV-2 exposure, symptoms and seroprevalence in healthcare workers in Sweden. ..." in: Nature communications, Vol. 11, Issue 1, pp. 5064, (2020) (PubMed).

Antigendetails

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Andere Bezeichnung: SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N Produkte)

Substanzklasse: Viral Protein

Hintergrund: Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology, 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.

Molekulargewicht: 47.3 kDa