GDNF Protein

Produktnummer ABIN6700947

Hersteller Produkt- Nr.: 012-001-v10-0010

Recombinant GDNF-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für GDNF Protein (ABIN6700947)

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Protein-Typ: Recombinant

Spezies: Ratte

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: 98,00%

Produktdetails

Hersteller Produkt- Nr.: 012-001-v10-0010

Hersteller: Rockland

Verwendungszweck: Rat Glial Derived Neurotrophic Factor Recombinant Protein

Aufreinigung: Glial Derived Neurotrophic Factor purity was determined to be greater than 98% as determined by analysis by reducing and non-reducing SDS-pAGE.

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent proliferation of C6 cells and is typically less than 0.1 μg/mL.

Anwendungsinformationen

Applikationshinweise:

Application Note: Glial Derived Neurotrophic Factor Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Glial Derived Neurotrophic Factor in immunological assays.

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Buffer:

Buffer: 0.01 M Sodium Phosphate, pH 7.5

Stabilizer: None

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Andere Bezeichnung: Gdnf

Hintergrund:

Synonyms: ATF-1

Background: Glial Cell Line-Derived Neurotrophic Factor (GDNF) is a neurotrophic factor that is closely related to other neurotrophic factors, such as Neurturin, Persephin, and Artemin, by a common structural feature called the cysteine-knot. GDNF signals through a multicomponent system of receptors that includes RET and GFRα1-4, to promote dopamine uptake, survival and differentiation of neurons. Recombinant rat GDNF is a non-glycosylated homodimer, containing two 135 amino acid chains, with a total molecular weight of 30.1 kDa.

UniProt: Q07731

Pathways: RTK Signalweg, Synaptic Membrane, Tube Formation, Autophagie, Smooth Muscle Cell Migration