CNTF Protein

Produktnummer ABIN6700904

Hersteller Produkt- Nr.: 009-001-u71-0005

Dieses Recombinant CNTF-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für CNTF Protein (ABIN6700904)

Target: CNTF (Ciliary Neurotrophic Factor (CNTF))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: 98,00%

Produktdetails

Hersteller Produkt- Nr.: 009-001-u71-0005

Hersteller: Rockland

Verwendungszweck: Human Ciliary Neurotrophic Factor Recombinant Protein

Aufreinigung: Ciliary Neurotrophic Factor purity was determined to be greater than 98% as determined by analysis by HpLC, UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent stimulation of TF-1 cells and is typically 0.1-0.6 ng/mL.

Anwendungsinformationen

Applikationshinweise:

Application Note: Ciliary Neurotrophic Factor Recombinant Protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-Ciliary Neurotrophic Factor in immunological assays.

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 5 μL (5-50 μL)

Buffer:

Buffer: 0.01 M Sodium Phosphate, pH 7.5

Stabilizer: None

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: CNTF (Ciliary Neurotrophic Factor (CNTF))

Andere Bezeichnung: CNTF

Hintergrund:

Synonyms: None

Background: Ciliary Neurotrophic Factor (CNTF) is a neurotrophic factor that promotes the survival of various neuronal cell types and may play an important role in the injury response in the nervous system. CNTF, like FGF acidic, FGF basic, and PD-ECGF (platelet-derived endothelial cell growth factor), does not possess a signal sequence that would allow secretion of the factor by classical secretion pathways (endoplasmatic reticulum/Golgi system), but the mechanism underlying the release of CNTF is unknown. Recombinant human CNTF is a non-glycosylated protein, contains 199 amino acids, with a molecular weight of 22.7 kDa.

UniProt: P26441

Pathways: JAK-STAT Signalweg