VEGFA Protein

Produktnummer ABIN6700875

Hersteller Produkt- Nr.: 012-001-b99-0010

Recombinant VEGFA-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für VEGFA Protein (ABIN6700875)

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Protein-Typ: Recombinant

Spezies: Ratte

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: 97,00%

Produktdetails

Hersteller Produkt- Nr.: 012-001-b99-0010

Hersteller: Rockland

Verwendungszweck: Rat Vascular Endothelial Growth Factor-165 Recombinant Protein

Aufreinigung: Vascular Endothelial Growth Factor-165 purity was determined to be greater than 97% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent induced proliferation of Human umbilical vein endothelial cells (HUVECs) and is typically 4.0-8.0 ng/mL.

Anwendungsinformationen

Applikationshinweise:

Application Note: Endothelial Growth Factor-165 Recombinant Protein has been tested by SDS-PAGE and bioactivity and is suitable as a control for polyclonal or monoclonal anti-Endothelial Growth Factor-165 in immunological assays.

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Buffer:

Buffer: 0.01 M Sodium Phosphate, pH 7.5

Stabilizer: None

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Andere Bezeichnung: Vegfa

Hintergrund:

Synonyms: VEGF-A, glioma-derived endothelial cell mitogen, Vascular permeability factor (VPF)

Background: Vascular Endothelial Growth Factor-A (VEGF-A) was originally isolated from tumor cells and is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability, VEGF-A may play a role in stimulating vasodilatation via nitric oxide-dependent pathways. VEGF-A has several variants, VEGF-165 being the most abundant. Rat and bovine VEGF are one amino acid shorter than the human factor, and the bovine and human sequences show a homology of 95 % . Recombinant rat VEGF-165 is a disulfide linked homodimer, containing two 165 amino acids chains, with a total molecular weight of 38.8 kDa.

UniProt: P16612-2

Pathways: RTK Signalweg, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling