VEGFA Protein

Produktnummer ABIN6700853

Hersteller Produkt- Nr.: 009-001-c23

Dieses Recombinant VEGFA-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für VEGFA Protein (ABIN6700853)

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: 95,00%

Produktdetails

Hersteller Produkt- Nr.: 009-001-c23

Hersteller: Rockland

Verwendungszweck: VEGF-121 Human Recombinant Protein

Aufreinigung: Purity was determined to be greater than 95% as determined by analysis by RP-HPLC, and by reducing and non-reducing SDS-PAGE, against known standard.

Endotoxin-Niveau: Measured by LAL is < 0.01 ng/μg or < 0.1 EU/μg protein.

Biological Activity Comment: The biological activity is determined by the dose- dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 0.1-0.4 ng/ml.

Anwendungsinformationen

Applikationshinweise:

Application Note: VEGF-121 protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-VEGF-121 in immunological assays.

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Konzentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Andere Bezeichnung: VEGFA

Hintergrund:

Synonyms: Vascular endothelial growth factor A-121, VEGF-A121 cytokine, Vascular permeability factor, VPF-121

Background: Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as Tumor Angiogenesis Factor. Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth, vascular permeability, cell migration, and endothelial cell proliferation and growth, it may play a role in stimulating vasolidation via nitric oxide-dependent pathways and in inhibition of apoptosis. VEGF binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Alternative splicing of the mRNA for VEGF-A results in several isoforms of the protein being produced. Rat and bovine VEGF are one amino acid shorter than the human factor, and the bovine and human sequences show a homology of 95 percent. In contrast to other factors mitogenic for endothelial cells such as FGF-1, FGF-2 and PDGF, VEGF is synthesized as a precursor containing a typical hydrophobic secretory signal sequence of 26 amino acids. Glycosylation is not required for efficient secretion of VEGF. VEGF121 is acidic, freely secreted, and widely expressed. This isoform is produced by alternative promoter usage and alternative initiation. It starts at an alternative upstream CUG codon and is post-translationally processed to produce the secreted VEGF peptide and a N-terminal peptide N-VEGF. The unprocessed protein and the N-VEGF peptide may localize to the nucleus, the endoplasmic reticulum and the Golgi or the extracellular matrix. Recombinant Human VEGF-121 produced in E.coli is a double, non-glycosylated, polypeptide chain containing 121 amino acids and having a molecular mass of 28423 Daltons.

UniProt: P15692-9

Pathways: RTK Signalweg, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling