TGFB1 Protein

Produktnummer ABIN6700722

Hersteller Produkt- Nr.: 009-001-w26-0005

Recombinant TGFB1-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für TGFB1 Protein (ABIN6700722)

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: 98,00%

Produktdetails

Hersteller Produkt- Nr.: 009-001-w26-0005

Hersteller: Rockland

Verwendungszweck: Human Transforming Growth Factor beta 1 Recombinant Protein

Aufreinigung: Transforming Growth Factor beta 1 purity was determined to be greater than 98% as determined by reducing and non-reducing SDS-pAGE.

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is measured by the dose-dependent inhibition of IL-4-induced proliferation from mouse HT-2 cells and is typically 0.04-0.2 ng/mL.

Anwendungsinformationen

Applikationshinweise:

Application Note: Transforming Growth Factor beta 1 Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Transforming Growth Factor beta 1 in immunological assays.

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 5 μL (5-50 μL)

Konzentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative, it does contain Trehalose in a ratio of 20:1 (Trehalose:Protein) DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Andere Bezeichnung: TGFB1

Hintergrund:

Synonyms: Differentiation inhibiting factor, cartilage-inducing factor

Background: Transforming Growth Factor-beta 1 (TGF-β1) is a member of the TGF-beta superfamily. Members of this family are known to exhibit regulatory activity in immunity and proliferation pathways. TGF-β1 signals through SMAD proteins via the TGF-β1 RI and RII receptors. Mature human TGF-β1 is a glycosylated homodimer, containing two 112 amino acids chains, with a total molecular weight of 25 kDa.

UniProt: P01137

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagie, Cancer Immune Checkpoints