Sonic Hedgehog Protein (SHH)

Produktnummer ABIN6700560

Hersteller Produkt- Nr.: 010-001-w24-0005

Recombinant Sonic Hedgehog-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für Sonic Hedgehog Protein (SHH) (ABIN6700560)

Target: Sonic Hedgehog (SHH)

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: 97,00%

Produktdetails

Hersteller Produkt- Nr.: 010-001-w24-0005

Hersteller: Rockland

Verwendungszweck: Mouse Sonic Hedgehog Recombinant Protein

Aufreinigung: Sonic Hedgehog purity was determined to be greater than 97% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is measured by the dose-dependent induction of alkaline phosphatase production by C3H/10T1/2 (CCL-226) fibroblasts and is typically 0.6-3 μg/mL.

Anwendungsinformationen

Applikationshinweise:

Application Note: Sonic Hedgehog Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Sonic Hedgehog in immunological assays.

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 5 μL (5-50 μL)

Buffer:

Buffer: 0.01 M Sodium Phosphate, pH 7.5

Stabilizer: None

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: Sonic Hedgehog (SHH)

Andere Bezeichnung: Shh

Hintergrund:

Synonyms: HHG-1

Background: Sonic hedgehog (SHH) is a member of a small group of secreted proteins that are essential for development in both vertebrates and invertebrates. Three mammalian hedgehog genes (sonic, desert, Indian) share about 60 % homology and all signal via the same receptors. Recombinant mouse SHH is a non-glycosylated protein, containing 176 amino acids, with a molecular weight of 19.8 kDa. The Cys at position 25 has been substituted with Ile.

UniProt: Q62226

Pathways: Hedgehog Signalweg, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development