IL-15 Protein

Produktnummer ABIN6700032

Hersteller Produkt- Nr.: 009-001-v53-0010

Recombinant IL-15-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für IL-15 Protein (ABIN6700032)

Target: IL-15 (IL15) (Interleukin 15 (IL15))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: 98,00%

Produktdetails

Hersteller Produkt- Nr.: 009-001-v53-0010

Hersteller: Rockland

Verwendungszweck: Human Interleukin-15 Recombinant Protein

Aufreinigung: Interleukin-15 purity was determined to be greater than 98% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependant stimulation of the proliferation of mouse CTLL-2 cells and is typcially less than 0.5 ng/mL.

Anwendungsinformationen

Applikationshinweise:

Application Note: Interleukin-15 Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Interleukin-15 in immunological assays. Lyophilized in 10 mM sodium bicarbonate, pH 8.5.

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Konzentration: 0.1 mg/mL

Buffer: Lyophilized in 10 mM sodium bicarbonate, pH 8.5.

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: IL-15 (IL15) (Interleukin 15 (IL15))

Andere Bezeichnung: IL15

Hintergrund:

Synonyms: IL-T

Background: Interleukin 15 (IL-15) is a widely expressed pro-inflammatory cytokine related to IL-2. IL-15 promotes activation of T cells, neutrophils and macrophages and is critical to dendritic cell function in several model systems. IL-15 has been shown to play a role in several inflammatory disorders, including rheumatoid arthritis, psoriasis and pulmonary inflammatory diseases. Emerging data suggests that IL-15 may serve as a good therapeutic target, as there appears to be a beneficial effect of IL-15 neutralization in models of psoriasis and diabetes. Human IL-15 shows activity on mouse cells. Recombinant human IL-15 is a non-glycosylated protein, containing 114 amino acids, with a molecular weight of 12.8 kDa.

UniProt: P40933

Pathways: JAK-STAT Signalweg, Glycosaminoglycan Metabolic Process