M-CSF/CSF1 Protein

Produktnummer ABIN6699714

Hersteller Produkt- Nr.: 010-001-v78-0100

Recombinant M-CSF/CSF1-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für M-CSF/CSF1 Protein (ABIN6699714)

Target: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: 98,00%

Produktdetails

Hersteller Produkt- Nr.: 010-001-v78-0100

Hersteller: Rockland

Verwendungszweck: Mouse Macrophage Colony Stimulating Factor Recombinant Protein

Aufreinigung: Macrophage Colony Stimulating Factor purity was determined to be greater than 98% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by dose-dependent induction of M-NFS-60 cell proliferation and is typically less than 2 ng/mL.

Anwendungsinformationen

Applikationshinweise:

Application Note: Macrophage Colony Stimulating Factor Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Macrophage Colony Stimulating Factor in immunological assays. Lyophilized in 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer: Lyophilized in 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Andere Bezeichnung: Csf1

Hintergrund:

Synonyms: MGI-IM

Background: Macrophage Colony Stimulating Factor (M-CSF) is hematopoietic growth factor produced by a wide variety of cells. M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells. Recombinant mouse M-CSF is a disulfide-linked homodimer, containing two 156 amino acid chains, with a total molecular weight of 36.8 kDa.

UniProt: P07141

Pathways: RTK Signalweg