CCL3 Protein

Produktnummer ABIN6699629

Hersteller Produkt- Nr.: 012-001-v80-0010

Dieses Recombinant CCL3-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für CCL3 Protein (ABIN6699629)

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Protein-Typ: Recombinant

Spezies: Ratte

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: 95,00%

Produktdetails

Hersteller Produkt- Nr.: 012-001-v80-0010

Hersteller: Rockland

Verwendungszweck: Rat Macrophage Inflammatory Protein-1 alpha (CCL3) Recombinant Protein

Aufreinigung: Macrophage Inflammatory protein-1 alpha (CCL3) purity was determined to be greater than 95% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the ability to chemoattract human PBMCs at 1-10 ng/mL.

Anwendungsinformationen

Applikationshinweise:

Application Note: Macrophage Inflammatory Protein-1 alpha Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Macrophage Inflammatory Protein-1 alpha in immunological assays.

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 20 μL (20-200 μL)

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Andere Bezeichnung: Ccl3

Hintergrund:

Synonyms: LD78α, Macrophage inflammatory protein 1-alpha (MIP-1-alpha)

Background: Macrophage Inflammatory Protein-1 alpha (MIP-1α), also known as CCL3, is produced by macrophages and is thought to induce inflammatory responses, including superoxide production by neutrophils. MIP-1α can exist as a naturally occurring heterodimer with MIP-1β and has been shown to have antiviral activity against HSV-1. Recombinant rat MIP-1α is a non-glycosylated protein, containing 69 amino acids, with a molecular weight of 7.8 kDa.

UniProt: P50229

Pathways: Cellular Response to Molecule of Bacterial Origin, Autophagie