CNTF Protein (AA 1-200)

Produktnummer ABIN6387771

Dieses Recombinant CNTF-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für CNTF Protein (AA 1-200) (ABIN6387771)

Target: CNTF (Ciliary Neurotrophic Factor (CNTF))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS), Activity Assay (AcA)

Reinheit: > 90 %

Produktdetails

Proteineigenschaft: AA 1-200

Sequenz: MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM

Aufreinigung: > 90% by SDS-PAGE

Endotoxin-Niveau: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤500ng/ml.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Bioactivity Validated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 10 % glycerol

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: CNTF (Ciliary Neurotrophic Factor (CNTF))

Andere Bezeichnung: CNTF

Hintergrund: CNTF (Ciliary neurotrophic factor) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. This protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. CNTF protein is structurally related to leukemia inhibitory factor (LIF), interleukin-6 (IL-6), interleukin-11 (IL-11) and oncostatin M (OSM). It is localized in the cell nucleus subsequent to receptor binding. Recombinant human CNTF protein was expressed in E. coli and purified by using conventional chromatography techniques.

Molekulargewicht: 22.9 kDa (200aa) confirmed by MALDI-TOF

NCBI Accession: NP_000605

Pathways: JAK-STAT Signalweg