SMAD3 Protein (His tag)
Kurzübersicht für SMAD3 Protein (His tag) (ABIN2940196)
Target
Alle SMAD3 Proteine anzeigenProtein-Typ
Spezies
Quelle
Applikation
Reinheit
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Aufreinigungstag / Konjugat
- Dieses SMAD3 Protein ist gelabelt mit His tag.
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Verwendungszweck
- Mouse Mothers Against Decapentaplegic Homolog 3 (Smad3) Protein
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Sequenz
- FWCSISYYEL NQRVGETFHA SQPSMTVDGF TDPSNSERFC LGLLSNVNRN AAVELTRRHI GRGVRLYYIG GEVFAECLSD SAIFVQSPNC NQRYGWHPAT VCKIPPGCNL KIFNNQEFAA LLAQSVNQGF EAVYQLTRMC TIRMSFVKGW GAEYRRQTVT STPCWIELHL NGPLQWLDKV LTQMGSPSIR CSSVS
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Biological Activity Comment
- Not tested
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SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 33-178) protein (His-IF2DI Tag)
SMAD3 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 1-425) protein (His tag)SMAD3 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant > 90 % SDS
SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 31-141) protein (GST tag)SMAD3 Spezies: Human Wirt: Wheat germ Recombinant AP, AA, ELISA, WB
SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 1-425) protein (His-GST)SMAD3 Spezies: Maus Wirt: Baculovirus infected Insect Cells Recombinant > 85 % as determined by reducing SDS-PAGE.
SMAD, Mothers Against DPP Homolog 3 (SMAD3) (Transcript Variant 1) (Active) protein (Myc-DYKDDDDK Tag)SMAD3 Spezies: Human Wirt: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, Func, PI, STD Active
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Kommentare
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Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire. -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- Prior to lyophilization: PBS, pH 7.4, containing 0.01 % Sarcosyl, 1 mM DTT, 5 % Trehalose and Proclin-300.
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Konservierungsmittel
- Dithiothreitol (DTT), ProClin
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Vorsichtsmaßnahmen
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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Lagerung
- 4 °C,-80 °C
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Informationen zur Lagerung
- Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.
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- SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
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Andere Bezeichnung
- Mothers Against Decapentaplegic Homolog 3
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Molekulargewicht
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Calculated MW: 23.2 kDa
Observed MW: 23 kDa -
UniProt
- Q8BUN5
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Pathways
- Zellzyklus, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagie
Target
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