EGFR Protein (AA 25-645) (His tag)

Produktnummer ABIN6253725

Dieses Recombinant EGFR-Protein wird in HEK-293 Cells exprimiert und wurde in 12+ Publikationen erwähnt.

Kurzübersicht für EGFR Protein (AA 25-645) (His tag) (ABIN6253725)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: >95 % as determined by SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 25-645

Aufreinigungstag / Konjugat: Dieses EGFR Protein ist gelabelt mit His tag.

Sequenz: AA 25-645

Produktmerkmale: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 69.5 kDa. The protein migrates as 110-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.

Referenzen

Haueis, Stech, Kubick: "A Cell-free Expression Pipeline for the Generation and Functional Characterization of Nanobodies." in: Frontiers in bioengineering and biotechnology, Vol. 10, pp. 896763, (2022) (PubMed).

Hu, Wang, Jin, Liu, Cui, Sun, Li, Li, Wang, Zhen, Miao, Li: "An EGFR-targeting antibody-drug conjugate LR004-VC-MMAE: potential in esophageal squamous cell carcinoma and other malignancies." in: Molecular oncology, Vol. 13, Issue 2, pp. 246-263, (2019) (PubMed).

Kahl, Settele, Knick, Haupts, Bosse-Doenecke: "Mabfilin and Fabfilin - New antibody-scaffold fusion formats for multispecific targeting concepts." in: Protein expression and purification, Vol. 149, pp. 51-65, (2018) (PubMed).

Fazekas-Singer, Singer, Ilieva, Matz, Herrmann, Spillner, Karagiannis, Jensen-Jarolim: "AllergoOncology: Generating a canine anticancer IgE against the epidermal growth factor receptor." in: The Journal of allergy and clinical immunology, Vol. 142, Issue 3, pp. 973-976.e11, (2018) (PubMed).

Fazekas-Singer, Berroterán-Infante, Rami-Mark, Dumanic, Matz, Willmann, Andreae, Singer, Wadsak, Mitterhauser, Jensen-Jarolim: "Development of a radiolabeled caninized anti-EGFR antibody for comparative oncology trials." in: Oncotarget, Vol. 8, Issue 47, pp. 83128-83141, (2017) (PubMed).

Yu, Pegram, Bigner, Chandramohan: "Development and validation of a cell-based fluorescent method for measuring antibody affinity." in: Journal of immunological methods, Vol. 442, pp. 49-53, (2017) (PubMed).

Nath, Flemming, Godat, Urh: "Development of NanoLuc bridging immunoassay for detection of anti-drug antibodies." in: Journal of immunological methods, Vol. 450, pp. 17-26, (2017) (PubMed).

Kant, Agarwal, Pancholi, Pancholi: "The Streptococcus pyogenes orphan protein tyrosine phosphatase, SP-PTP, possesses dual specificity and essential virulence regulatory functions." in: Molecular microbiology, Vol. 97, Issue 3, pp. 515-40, (2016) (PubMed).

Zinn, Korb, Samuel, Warram, Dion, Killingsworth, Fan, Schoeb, Strong, Rosenthal: "IND-directed safety and biodistribution study of intravenously injected cetuximab-IRDye800 in cynomolgus macaques." in: Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging, Vol. 17, Issue 1, pp. 49-57, (2015) (PubMed).

Olsen, Jakobsen, Brandslund: "Quantification of EGFR autoantibodies in the amplification phenomenon of HER2 in breast cancer." in: Clinical chemistry and laboratory medicine, Vol. 51, Issue 12, pp. 2325-9, (2014) (PubMed).

Singer, Fazekas, Wang, Weichselbaumer, Matz, Mader, Steinfellner, Meitz, Mechtcheriakova, Sobanov, Willmann, Stockner, Spillner, Kunert, Jensen-Jarolim: "Generation of a canine anti-EGFR (ErbB-1) antibody for passive immunotherapy in dog cancer patients." in: Molecular cancer therapeutics, Vol. 13, Issue 7, pp. 1777-90, (2014) (PubMed).

Antigendetails

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Andere Bezeichnung: EGF R

Hintergrund: The epidermal growth factor receptor (EGFR, ErbB-1, HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.

Molekulargewicht: 70.5kDa

NCBI Accession: NP_005219

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteine