FGF1 Protein (AA 1-155) (His tag,GST tag)

Produktnummer ABIN6239784

Dieses FGF1-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für FGF1 Protein (AA 1-155) (His tag,GST tag) (ABIN6239784)

Target: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Activity Assay (AcA), Cell Culture (CC)

Reinheit: > 97 %

Produktdetails

Proteineigenschaft: AA 1-155

Aufreinigungstag / Konjugat: Dieses FGF1 Protein ist gelabelt mit His tag,GST tag.

Produktmerkmale: Tag location: N-terminal His and GST Tag

Biological Activity Comment: FGF1 (Fibroblast growth factor 1) is a member of FGF family, which plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. FGF1 is thought to stimulate the proliferation of 3T3 fibroblasts, besides, human FGF1 and mouse FGF1 shares similarities in amino acid sequence with the identity of 96%. Thus, a cell proliferation assay was conducted to detect the bioactivity of recombinant human FGF1 using 3T3 fibroblasts. Briefly, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of FGF1. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of 3T3 cells after incubation with FGF1 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant FGF1 for 48h. The result was shown in Figure 2. It was obvious that FGF1 significantly increased cell viability of 3T3 cells.

Anwendungsinformationen

Applikationshinweise: Isoelectric Point: 6.5

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Konservierungsmittel: Dithiothreitol (DTT), Other preservative, ProClin

Vorsichtsmaßnahmen: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Antigendetails

Target: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Andere Bezeichnung: Fibroblast Growth Factor 1, Acidic (FGF1)

Hintergrund: Alternative Names: ECGF, AFGF, ECGFA, ECGFB, FGF-Alpha, FGFA, HBGF1, ECGFB, HBGF1, Acidic Fibroblast Growth Factor, heparin-binding growth factor 1, Endothelial Cell Growth Factor, Beta

Molekulargewicht: 55kDa

UniProt: P05230

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung