Leptin Protein (LEP) (His tag)
Kurzübersicht für Leptin Protein (LEP) (His tag) (ABIN6124311)
Target
Alle Leptin (LEP) Proteine anzeigenProtein-Typ
Spezies
Quelle
Applikation
Reinheit
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Aufreinigungstag / Konjugat
- Dieses Leptin Protein ist gelabelt mit His tag.
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Verwendungszweck
- Dog Leptin (LEP) Protein
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Sequenz
- VPIRKVQDD TKTLIKTIVA RINDISHTQS VSSKQRVAGL DFIPGLQPVL SLSRMDQTLA IYQQILNSLH SRNVVQISND LENLRDLLHL LASSKSCPLP RARGLETFES LGGVLEASLY STEVVALNRL QAALQDMLRR LDLSPGC
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Endotoxin-Niveau
- < 1.0 EU per 1 μg (determined by the LAL method)
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Biological Activity Comment
- Not tested
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Leptin (LEP) (AA 22-167) (Active) protein (hFc Tag)
LEP Spezies: Maus Wirt: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Leptin (LEP) (AA 22-167) protein (His-IF2DI Tag)LEP Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Leptin (LEP) (AA 22-167) protein (His tag)LEP Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Leptin (LEP) (AA 22-167) protein (His tag)LEP Spezies: Schwein Wirt: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Leptin (LEP) (AA 428-635) protein (His-IF2DI Tag)LEP Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Leptin (LEP) (AA 22-167) protein (Fc Tag)LEP Spezies: Human Wirt: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Leptin (LEP) (AA 22-171) protein (His tag)LEP Spezies: Crucian Carp Wirt: Yeast (Pichia pastoris) Recombinant > 95 % as determined by reducing SDS-PAGE.
Leptin (LEP) (AA 22-167) protein (GST tag)LEP Spezies: Human Wirt: Wheat germ Recombinant AP, AA, ELISA, WB
Leptin (LEP) (AA 22-167) (Active) proteinLEP Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE. ELISA, BLI Active
Leptin (LEP) (AA 22-167), (full length) protein (mFc Tag)LEP Spezies: Schwein Wirt: Mammalian Cells Recombinant Greater than 95 % as determined by SDS-PAGE.
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Kommentare
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Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire. -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01 % Sarcosyl, 5 % Trehalose.
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Lagerung
- 4 °C,-80 °C
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Informationen zur Lagerung
- Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.
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- Leptin (LEP)
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Andere Bezeichnung
- Leptin
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Molekulargewicht
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Calculated MW: 19.8 kDa
Observed MW (SDS-PAGE): 20 kDa -
UniProt
- O02720
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Pathways
- JAK-STAT Signalweg, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
Target
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