EPO Protein (His tag)

Produktnummer ABIN6122629

Recombinant EPO-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für EPO Protein (His tag) (ABIN6122629)

Target: EPO (Erythropoietin (EPO))

Protein-Typ: Recombinant

Spezies: Affe

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS), Western Blotting (WB)

Reinheit: > 95 %

Produktdetails

Aufreinigungstag / Konjugat: Dieses EPO Protein ist gelabelt mit His tag.

Verwendungszweck: Monkey Erythropoietin (EPO) Protein

Sequenz: APP RLICDSRVLE RYLLEAKEAE NVTMGCSESC SLNENITVPD TKVNFYAWKR MEVGQQAVEV WQGLALLSEA VLRGQAVLAN SSQPFEPLQL HMDKAISGLR SITTLLRALG AQQEAISLPD AASAAPLRTI TADTFCKLFR VYSNFLRGKL KLYTGEACRR GDR

Endotoxin-Niveau: < 1.0 EU per 1 μg (determined by the LAL method)

Biological Activity Comment: Not tested

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.

Buffer: Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01 % Sarcosyl, 5 % Trehalose.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.

Antigendetails

Target: EPO (Erythropoietin (EPO))

Andere Bezeichnung: Erythropoietin

Substanzklasse: Hormone

Molekulargewicht: Calculated MW: 22.1 kDa
Observed MW (SDS-PAGE): 27 kDa

Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.

Gen-ID

719294

UniProt: Q28513

Pathways: JAK-STAT Signalweg, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity