EGFR Protein (AA 25-645) (His tag,AVI tag,Biotin)

Produktnummer ABIN5674594

Dieses Recombinant EGFR-Protein wird in HEK-293 Cells produziert. Es sind 3 Publikationen verfügbar.

Kurzübersicht für EGFR Protein (AA 25-645) (His tag,AVI tag,Biotin) (ABIN5674594)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: >90 % as determined by SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 25-645

Aufreinigungstag / Konjugat: Dieses EGFR Protein ist gelabelt mit His tag,AVI tag,Biotin.

Marke: PrecisionAvi

Sequenz: AA 25-645

Spezifität: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Produktmerkmale: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 72.3 kDa. The protein migrates as 100-125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Kommentare:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Referenzen

Tang, Zheng, Melo, Mabardi, Castaño, Xie, Li, Kudchodkar, Wong, Jeng, Maus, Irvine: "Enhancing T cell therapy through TCR-signaling-responsive nanoparticle drug delivery." in: Nature biotechnology, Vol. 36, Issue 8, pp. 707-716, (2019) (PubMed).

Choi, Yu, Castano, Bouffard, Schmidts, Larson, Bailey, Boroughs, Frigault, Leick, Scarfò, Cetrulo, Demehri, Nahed, Cahill, Wakimoto, Curry, Carter, Maus: "CAR-T cells secreting BiTEs circumvent antigen escape without detectable toxicity." in: Nature biotechnology, (2019) (PubMed).

Yu, Pegram, Bigner, Chandramohan: "Development and validation of a cell-based fluorescent method for measuring antibody affinity." in: Journal of immunological methods, Vol. 442, pp. 49-53, (2017) (PubMed).

Antigendetails

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Andere Bezeichnung: EGF R

Hintergrund: The epidermal growth factor receptor (EGFR, ErbB-1, HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.

Molekulargewicht: 72.3 kDa

NCBI Accession: NP_005219

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteine