CD14 Protein (CD14) (His tag)

Produktnummer ABIN5663350

Dieses Recombinant CD14-Protein wird in CHO Cells produziert.

Kurzübersicht für CD14 Protein (CD14) (His tag) (ABIN5663350)

Target: CD14

Protein-Typ: Recombinant

Spezies: Maus

Quelle: CHO Cells

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Agonist (Agon)

Reinheit: >95 % by SDS-PAGE

Produktdetails

Aufreinigungstag / Konjugat: Dieses CD14 Protein ist gelabelt mit His tag.

Sequenz: SPAPPEPCEL DEESCSCNFS DPKPDWSSAF NCLGAADVEL YGGGRSLEYL LKRVDTEADL GQFTDIIKSL SLKRLTVRAA RIPSRILFGA LRVLGISGLQ ELTLENLEVT GTAPPPLLEA TGPDLNILNL RNVSWATRDA WLAELQQWLK PGLKVLSIAQ AHSLNFSCEQ VRVFPALSTL DLSDNPELGE RGLISALCPL KFPTLQVLAL RNAGMETPSG VCSALAAARV QLQGLDLSHN SLRDAAGAPS CDWPSQLNSL NLSFTGLKQV PKGLPAKLSV LDLSYNRLDR NPSPDELPQV GNLSLKGNPF LDSESHSEKF NSGVVTAGAP SSQAVALSGT LALLLGDRLF VHHHHHH

Produktmerkmale: The recombinant mouseCD14 is produced from mouse CD14 transfected CHO-cells in serum free medium, has been purified by His-tag with Talon (Clontech) and controlled by SDS page. Before transfection the complete mouse CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR.

Aufreinigung: Affinity chromatography by His-Tag

Endotoxin-Niveau: <1 EU/μgof recombinant mouse CD14 as determined by LAL method

Biological Activity Comment: Up to 5μg/ml mouse CD14 inhibit binding of FITC-LPS (0.5μg/ml) to mouse CD14+CHO transfectants, don't activate monocytes itself

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 40 μL sterile distilled water to a concentration of 0.25 mg/mL

Konzentration: 0.25 mg/mL

Buffer: sterile-filtered phosphate balanced salt solution, pH 7.5

Konservierungsmittel: Without preservative

Lagerung: RT,4 °C,-20 °C,-80 °C

Informationen zur Lagerung:

Storage: -20°C or -70°C

Storage/Stability: Long time storage at -70oC. When stored at recommended temperature, this protein is stable for 12 months.The lyophilized product thought stable at -70oC more than 1 year, at refrigerator about 10 months at room temperature up to 4 weeks, and at 37oC 1 week. Reconstituted protein should be used immediately or stored in undiluted working aliquots at -20oC. For long term storage it is recommended to add a carrier protein (o.1% BSA). Repeated freezing and thawing should be avoided.

Haltbarkeit: 12 months

Antigendetails

Target: CD14

Andere Bezeichnung: CD14

Hintergrund: Background: CD14 acts as a receptor for endotoxin (LPS), expressed strongly on monocytes, macro-phages and weekly at surface of neutrophiles. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor LPS-LBP (lipopolysaccharide binding protein)-complexes. CD14 cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.CD14 is expressed strongly on the surface of monocytes and weakly on the surface of granulocytes, also expressed by most tissue macrophages.

Molekulargewicht: ~50kDa

Gen-ID: 12475

NCBI Accession: NP_033971

UniProt: P10810

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades