CD14 Protein (CD14) (His tag)

Produktnummer ABIN5663349

Recombinant CD14-Protein exprimiert in CHO Cells.

Kurzübersicht für CD14 Protein (CD14) (His tag) (ABIN5663349)

Target: CD14

Protein-Typ: Recombinant

Spezies: Human

Quelle: CHO Cells

Applikation: Western Blotting (WB), ELISA, Agonist (Agon), Dot Blot (DB)

Reinheit: >95 % by SDS-PAGE

Produktdetails

Aufreinigungstag / Konjugat: Dieses CD14 Protein ist gelabelt mit His tag.

Sequenz: MERASCLLLL LLPLVHVSAT TPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QPHSLDLSHN SLRATVNPSA PRCMWSSALN SLNLSFAGLE QVPKGLPAKL RVLDLSCNRL NRAPQPDELP EVDNLTLDGN PFLVPGTALP HEGSMNSGVV PACARSTLSV G VSGTLVLLQG ARGFA

Produktmerkmale: The hCD14 is produced from human CD14 transfected CHO-cells. Before transfection the complete human CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR.

Aufreinigung: affinity chromatography with monoclonal CD14 antibody biG2

Endotoxin-Niveau: <1EU/μg of recombinant human CD14 as determined by LAL method

Biological Activity Comment: Up to 20 μg/ml CD14 inhibit binding of FITC-LPS (0.5μg/ml) to 6 x 105 CD14+CHO transfectants (FACS)

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 40 μL sterile distilled water to a concentration of 0.25 mg/mL

Konzentration: 0.25 mg/mL

Buffer: sterile-filtered phosphate balanced salt solution, pH 7.2

Konservierungsmittel: Without preservative

Lagerung: RT,-20 °C,-80 °C

Informationen zur Lagerung:

Storage: -20°C or -70°C

Storage/Stability: Long time storage at -70oC. When stored at recommended temperature, this protein is stable for 12 months. The lyophilized product thought stable room temperature up to 3 weeks, but is best stored desiccated at -70oC more than 1 year, at refrigerator about 10 months. Reconstituted protein should be used immediately or stored in undiluted working aliquots at -20oC. For long-term storage it is recommended to add a carrier protein (o.1% BSA). Repeated freezing and thawing should be avoided !!!

Haltbarkeit: 12 months

Antigendetails

Target: CD14

Andere Bezeichnung: CD14

Hintergrund: Background: CD14 acts as a receptor for endotoxin (LPS), expressed strongly on monocytes, macrophages and weekly at surface of neutrophiles. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor LPS-LBP (lipopolysaccharide binding protein)-complexes. CD14 cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.The rhuCD14 was produced in human CD14 transfected CHO-cells in serum free medium and has been purified by affinity chromatography with monoclonal antibody to hCD14 biG2at column and controlled by SDS page (90-95 % purity). Before transfection, the complete human CD14-cDNA amplified by PCR and cloned into expression vector p-POL-DHFR. CD14 is expressed strongly on the surface of monocytes and weakly on the surface of granulocytes, also expressed by most tissue macrophages.

Molekulargewicht: ~50kDa

Gen-ID: 929

NCBI Accession: NP_000582

UniProt: P08571

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades