FASL Protein (AA 18-111, AA 139-281) (DYKDDDDK Tag)
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- Target Alle FASL Proteine anzeigen
- FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
- Proteineigenschaft
- AA 139-281, AA 18-111
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Spezies
- Human, Maus
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Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- Dieses FASL Protein ist gelabelt mit DYKDDDDK Tag.
- Applikation
- SDS-PAGE (SDS)
- Verwendungszweck
- FasL (human) (multimeric) (rec.)
- Spezifität
- Binds to human and mouse Fas.
- Kreuzreaktivität
- Human, Maus
- Produktmerkmale
- Human FasL (aa 139-281) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag.
- Reinheit
- >95 % (SDS-PAGE)
- Endotoxin-Niveau
- <0.02EU/μg purified protein (LAL test).
- Biological Activity Comment
- Induces apoptosis of human Jurkat T cells at a concentration of <0.2ng/ml.
- Top Product
- Discover our top product FASL Protein
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Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 1-281) protein (His tag)
custom-made FASL Spezies: Human Wirt: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 134-281) (Active) protein (His tag)FASL Spezies: Human Wirt: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 1-281) protein (GST tag)FASL Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AP, AA
Fas Ligand (TNF Superfamily, Member 6) (FASL) proteinFASL Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant > 90 % pure SDS
Fas Ligand (TNF Superfamily, Member 6) (FASL) protein (Fc Tag)FASL Spezies: Human Wirt: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Konzentration
- 0.1 mg/mL
- Buffer
- Contains PBS.
- Handhabung
- After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
- Haltbarkeit
- 6 months
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- Target
- FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))
- Andere Bezeichnung
- FasL (FASL Produkte)
- Hintergrund
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MultimericFasL™, MegaFasL, ACRP30headless:APO-1L, ACRP30headless:CD95L, ACRP30headless:CD178, ACRP30headless:TNFSF6, ACRP30headless:Fas Ligand
MultimericFasL™ is a high activity construct in which two trimeric FasL are artificially linked via the collagen domain of ACRP30. This construct very effectively mimics the natural membrane-assisted aggregation of FasL in vivo. FasL is a cytokine that binds to TNFRSF6/Fas, a receptor that transduces the apoptotic signal into cells. It is involved in cytotoxic T cell mediated apoptosis and in T cell development. FasL treatment of stem cells is a robust method for proliferating cells in culture while improving their stemness properties. Treatment with FasL results in a large quantity of Adipose Derived Stem Cells (ASC) of high quality.
- Molekulargewicht
- ~40kDa (SDS-PAGE)
- UniProt
- P48023
- Pathways
- Apoptose, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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