SLC16A1 Protein (AA 1-493) (Strep Tag)

Produktnummer ABIN3134481

Produktdetails

Target: SLC16A1 (Solute Carrier Family 16, Member 1 (Monocarboxylic Acid Transporter 1) (SLC16A1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-493

Spezies: Maus

Quelle: Cell-free protein synthesis (CFPS)

Aufreinigungstag / Konjugat: Dieses SLC16A1 Protein ist gelabelt mit Strep Tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Marke: AliCE®

Sequenz: MPPAIGGPVG YTPPDGGWGW AVLVGAFISI GFSYAFPKSI TVFFKEIEVI FSATTSEVSW ISSIMLAVMY AGGPISSILV NKYGSRPVMI AGGCLSGCGL IAASFCNTVQ ELYLCIGVIG GLGLAFNLNP ALTMIGKYFY KKRPLANGLA MAGSPVFLST LAPLNQAFFD IFDWRGSFLI LGGLLLNCCV AGSLMRPIGP EQVKLEKLKS KESLQEAGKS DANTDLIGGS PKGEKLSVFQ TINKFLDLSL FTHRGFLLYL SGNVVMFFGL FTPLVFLSSY GKSKDFSSEK SAFLLSILAF VDMVARPSMG LAANTKWIRP RIQYFFAASV VANGVCHLLA PLSTTYVGFC VYAGVFGFAF GWLSSVLFET LMDLIGPQRF SSAVGLVTIV ECCPVLLGPP LLGRLNDMYG DYKYTYWACG VILIIAGIYL FIGMGINYRL LAKEQKAEEK QKREGKEDEA STDVDEKPKE TMKAAQSPQQ HSSGDPTEEE SPV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).

Reinheit: > 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Güteklasse: custom-made

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: 12 months

Antigendetails

Target: SLC16A1 (Solute Carrier Family 16, Member 1 (Monocarboxylic Acid Transporter 1) (SLC16A1))

Andere Bezeichnung: Slc16a1 (SLC16A1 Produkte)

Hintergrund: Monocarboxylate transporter 1 (MCT 1) (Solute carrier family 16 member 1),FUNCTION: Bidirectional proton-coupled monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, acetate and the ketone bodies acetoacetate and beta-hydroxybutyrate, and thus contributes to the maintenance of intracellular pH (By similarity). The transport direction is determined by the proton motive force and the concentration gradient of the substrate monocarboxylate. MCT1 is a major lactate exporter (By similarity). Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis (PubMed:22522610, PubMed:24367518). Facilitates the protonated monocarboxylate form of succinate export, that its transient protonation upon muscle cell acidification in exercising muscle and ischemic heart. Functions via alternate outward- and inward-open conformation states. Protonation and deprotonation of 302-Asp is essential for the conformational transition (PubMed:32946811). {ECO:0000250|UniProtKB:P53985, ECO:0000250|UniProtKB:P53987, ECO:0000269|PubMed:22522610, ECO:0000269|PubMed:24367518, ECO:0000269|PubMed:32946811}.

Molekulargewicht: 53.3 kDa

UniProt: P53986

Pathways: Warburg Effekt