FZD5 Protein (AA 27-180) (Fc Tag)

Produktnummer ABIN2745685

Produktdetails

Target: FZD5 (Frizzled Family Receptor 5 (FZD5))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 27-180

Spezies: Human, Maus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses FZD5 Protein ist gelabelt mit Fc Tag.

Applikation: SDS-PAGE (SDS)

Verwendungszweck: Frizzled-5 (human):Fc (human) (rec.)

Spezifität: Binds to human and mouse Wnt3a.

Kreuzreaktivität: Human, Maus

Produktmerkmale: The cysteine-rich domain (CRD) of human Frizzled-5 (aa 27-180) is fused at the C-terminus to the Fc portion of human IgG1.

Reinheit: >95 % (SDS-PAGE)

Endotoxin-Niveau: <0.01EU/μg purified protein (LAL test).

Biological Activity Comment: Inhibits Wnt3a activation in a stoechiometric manner.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 50 μL sterile water.

Konzentration: 1 mg/mL

Buffer: Contains PBS.

Handhabung: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Haltbarkeit: 6 months

Antigendetails

Target: FZD5 (Frizzled Family Receptor 5 (FZD5))

Andere Bezeichnung: Frizzled-5 (FZD5 Produkte)

Hintergrund:

FzE5, Fz-5, hFz5

Wnts are lipid-modified morphogens that control many processes during development, stem cell maintenance and homeostasis and their aberrant regulation has been linked to diseases in man including diabetes, neurodegeneration and cancer. Three distinct receptor families mediate Wnt signaling: Fzs, the single-pass LRP5/6 co-receptors and the atypical tyrosine kinase receptors Ror2 and Ryk. Frizzled proteins can activate canonical Wnt/beta-catenin signaling as well as non-canonical pathways (Wnt/Ca2+ pathways and planar cell polarity). The cysteine-rich domain (CRD) of the frizzled receptors situated on the extracellular part of the receptor is sufficient to bind the Wnt ligands.

Molekulargewicht: ~50kDa (SDS-PAGE)

UniProt: Q13467

Pathways: WNT Signalweg