VEGFA
Spezies: Ratte
Wirt: Escherichia coli (E. coli)
Recombinant
> 95 % as determined by SDS-PAGE and Coomassie blue staining
Func, AbP, STD, PI
Active
VEGFA
(Vascular Endothelial Growth Factor A (VEGFA))
Protein-Typ
Recombinant
Biologische Aktivität
Active
Proteineigenschaft
Transcript Variant 5
Spezies
Ratte
Quelle
Escherichia coli (E. coli)
Applikation
Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)
Spezifität
Optimal preservation of protein structure, post-translational modifications and functions.
Produktmerkmale
Recombinant human Vascular endothelial growth factor A (Vegfa), transcript variant 5. (transcript variant 5) protein expressed in E. coli.
Produced with end-sequenced ORF clone
Tested for bioactivity.
Reinheit
> 95 % as determined by SDS-PAGE and Coomassie blue staining
Endotoxin-Niveau
Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
Biological Activity Comment
Determined by the dose-dependent stimulation of the proliferation of Human umbilical vein endothelial cells (HUVEC). The expected ED50 is 4.0-8.0 ng/ml.
VEGFA
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
VEGFA
Spezies: Human
Wirt: Wheat germ
Recombinant
WB, ELISA, AA, AP
Applikationshinweise
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
Handhabung
Resuspend the protein in the desired concentration in proper buffer
Lagerung
-80 °C
Informationen zur Lagerung
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
Target
VEGFA
(Vascular Endothelial Growth Factor A (VEGFA))