VEGFA Protein (Transcript Variant 5)

Produktnummer ABIN2735180

Recombinant VEGFA-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für VEGFA Protein (Transcript Variant 5) (ABIN2735180)

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Ratte

Quelle: Escherichia coli (E. coli)

Applikation: Antibody Production (AbP), Functional Studies (Func), Standard (STD), Protein Interaction (PI)

Reinheit: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Produktdetails

Proteineigenschaft: Transcript Variant 5

Spezifität: Optimal preservation of protein structure, post-translational modifications and functions.

Produktmerkmale:

• Recombinant human Vascular endothelial growth factor A (Vegfa), transcript variant 5. (transcript variant 5) protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Endotoxin-Niveau: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: Determined by the dose-dependent stimulation of the proliferation of Human umbilical vein endothelial cells (HUVEC). The expected ED50 is 4.0-8.0 ng/ml.

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

Handhabung: Resuspend the protein in the desired concentration in proper buffer

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Molekulargewicht: 38.5 kDa

NCBI Accession: NP_001103806

Pathways: RTK Signalweg, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling