RAC1 Protein (Myc-DYKDDDDK Tag)

Produktnummer ABIN2730391

Dieses Recombinant RAC1-Protein wird in HEK-293 Cells exprimiert.

Kurzübersicht für RAC1 Protein (Myc-DYKDDDDK Tag) (ABIN2730391)

Target: RAC1

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Applikation: Antibody Production (AbP), Standard (STD)

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Produktdetails

Aufreinigungstag / Konjugat: Dieses RAC1 Protein ist gelabelt mit Myc-DYKDDDDK Tag.

Produktmerkmale:

• Recombinant human RAC1 (transcript variant Rac1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Kommentare:

The tag is located at the C-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: RAC1

Hintergrund: Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction. [UniProtKB/Swiss-Prot Function]

Molekulargewicht: 21.3 kDa

NCBI Accession: NP_008839

Pathways: WNT Signalweg, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Thromboxane A2 Receptor Signaling, VEGF Signaling