MGMT Protein (Myc-DYKDDDDK Tag)

Produktnummer ABIN2727841

Dieses Recombinant MGMT-Protein wird in HEK-293 Cells exprimiert.

Kurzübersicht für MGMT Protein (Myc-DYKDDDDK Tag) (ABIN2727841)

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Applikation: Antibody Production (AbP), Standard (STD)

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Produktdetails

Aufreinigungstag / Konjugat: Dieses MGMT Protein ist gelabelt mit Myc-DYKDDDDK Tag.

Produktmerkmale:

• Recombinant human O-6-methylguanine-DNA methyltransferase (MGMT) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Kommentare:

The tag is located at the C-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Andere Bezeichnung: O-6-Methylguanine-Dna Methyltransferase (Mgmt)

Hintergrund: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. [UniProtKB/Swiss-Prot Function]

Molekulargewicht: 24.9 kDa

NCBI Accession: NP_002403

Pathways: DNA Reparatur, Positive Regulation of Response to DNA Damage Stimulus