MGMT Protein (His tag)

Produktnummer ABIN7318122

Dieses Recombinant MGMT-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für MGMT Protein (His tag) (ABIN7318122)

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: > 90 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses MGMT Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human MGMT Protein (His Tag)

Sequenz: Met 1-Asn207

Produktmerkmale: Recombinant Human Methylated-DNA--protein-cysteine methyltransferase is produced by our E.coli expression system and the target gene encoding Met1-Asn207 is expressed with a 6His tag at the N-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl,1 mM DTT,1 mM EDTA,500 mM NaCl,0.1 % Trition X-100, pH 8.0.

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Andere Bezeichnung: MGMT

Hintergrund: 6-O-methylguanine-DNAmethyltransferase,MGMT,Methylated-DNA--protein-cysteine methyltransferase,O-6-methylguanine-DNA-alkyltransferase,MGMT belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. MGMT involved in the cellular defense against the biological effects of O6-methylguanine in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. MGMT catalyzes the chemical reaction: DNA (containing 6-O-methylguanine) and proteinL-cysteine into DNA (without 6-O-methylguanine) and protein S-methyl-L-cysteine.

Molekulargewicht:

Calculated MW: 23.8 kDa

Observed MW: 25 kDa

Gen-ID: 4255

UniProt: P16455

Pathways: DNA Reparatur, Positive Regulation of Response to DNA Damage Stimulus