Functional Studies (Func), Standard (STD), Antibody Production (AbP), Protein Interaction (PI)
Spezifität
Optimal preservation of protein structure, post-translational modifications and functions.
Produktmerkmale
Recombinant human M-CSF / CSF-1 (transcript variant 3) protein expressed in HEK293.
Produced with end-sequenced ORF clone
Tested for bioactivity.
Reinheit
> 98 % , as determined by Coomassie stained SDS-PAGE.
Endotoxin-Niveau
Less than 0.01 ng per μg protein as determined by the LAL method.
Biological Activity Comment
The ED50 is 2 - 6 ng/ml, corresponding to a specific activity of 1.6 - 5 x 10^5 units/mg, determined by M-NFS60 cell proliferation induced by mouse M-CSF in a dose dependent manner.
CSF1
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
>90 % as determined by SDS-PAGE.
Active
Applikationshinweise
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
> 50 μg/mL
Buffer
1 x PBS
Lagerung
-80 °C
Informationen zur Lagerung
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.