IL-15 Protein (Transcript Variant 3)

Produktnummer ABIN2723622

Dieses Recombinant IL-15-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für IL-15 Protein (Transcript Variant 3) (ABIN2723622)

Target: IL-15 (IL15) (Interleukin 15 (IL15))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Functional Studies (Func), Standard (STD), Antibody Production (AbP), Protein Interaction (PI)

Reinheit: Purity is > 98 % , as determined by Coomassie stained SDS-PAGE.

Produktdetails

Proteineigenschaft: Transcript Variant 3

Spezifität: Optimal preservation of protein structure, post-translational modifications and functions.

Produktmerkmale:

• Recombinant human Interleukin-15 / IL15 (transcript variant 3) protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Endotoxin-Niveau: Less than 0.01 ng per ug protein as determined by the LAL method.

Biological Activity Comment: The ED50 is 0.10-0.50 ng/ml, corresponding to a specific activity of 2-10 x 10^6 units/mg, determined by the dose dependent stimulation of CTLL-2 cells proliferation.

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: > 50 μg/mL

Buffer: 10 mM NaH2PO4, 150 mM NaCl, pH 7.2

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: IL-15 (IL15) (Interleukin 15 (IL15))

Andere Bezeichnung: Interleukin-15,il15

Hintergrund: Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha. [UniProtKB/Swiss-Prot Function]

Molekulargewicht: 12.8 kDa

NCBI Accession: NP_000576

Pathways: JAK-STAT Signalweg, Glycosaminoglycan Metabolic Process