VEGFA Protein (AA 27-190)

Produktnummer ABIN2666576

Dieses Recombinant VEGFA-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für VEGFA Protein (AA 27-190) (ABIN2666576)

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Ratte

Quelle: HEK-293 Cells

Applikation: Flow Cytometry (FACS)

Reinheit: > 95 % , as determined by Coomassie stained SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 27-190

Sterilität: 0.22 μm filtered

Endotoxin-Niveau:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: ED50 = 0.6 - 3.6 ng/mL, corresponding to a specific activity of 0.27 - 1.67 x 106 units/mg, as determined by induction of HUVEC cell proliferation in a dose-dependent manner.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20 °C to -70 °C. Stock solutions can also be prepared at 50 - 100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2 - 1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in 5 mM citric acid, 5 mM NaHPO4, 0.15 M NaCl, pH 4.0.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Antigendetails

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Andere Bezeichnung: VEGF-164

Hintergrund: The VEGF family includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, and placental growth factor (PlGF). VEGF-A is generated as multiple isoforms by alternative splicing. These isoforms are generically identified as VEGFxxx where xxx refers to the number of amino acids. Rat isoforms include VEGF-120, VEGF-164, and VEGF-188. The VEGF gene is highly conserved among human, dog, cow, mouse, rat, pig, and chicken. VEGF164 plays a critical role in new blood vessel formation in vivo. VEGF164 induces angiogenesis on different levels. It acts as mitogen especially on endothelial cells, raises the vessel permeability and dilatation by releasing NO, and has chemotactic impact on monocytes/macrophages which plays a crucial role in inducing inflammatory neovascularization. Angiogenic activities of VEGFs are mediated primarily through the two receptors VEGFR-1 and VEGFR-2. VEGFR-2 signaling is enhanced by interactions with co-receptors such as heparin/heparan sulfate and Neuropilin 1. VEGF induces osteoclast migration and activation. In addition, it supports the survival of mature osteoclasts. These processes play a key role in repairing and remodeling during bone development.

Molekulargewicht: The 164 amino acid recombinant protein has a predicted molecular mass of approximately 19.2 kDa. The protein migrates approximately at 25 kDa in DTT-reducing conditions and at approximately 50 kDa in non-reducing conditions by SDS-PAGE. The predicted N-te

Pathways: RTK Signalweg, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling