Prolactin Protein (PRL) (AA 29-227, N-Term)

Produktnummer ABIN2666567

Dieses Recombinant Prolactin-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für Prolactin Protein (PRL) (AA 29-227, N-Term) (ABIN2666567)

Target: Prolactin (PRL)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Flow Cytometry (FACS)

Reinheit: > 95 % , as determined by Coomassie stained SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 29-227, N-Term

Sterilität: 0.22 μm filtered

Endotoxin-Niveau:

Less than 0.1 EU per μg of protein as determined by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: Human Prolactin induces proliferation of MCF7 breast cancer cells in a dose dependent manner. The ED50 is 60 - 350 ng/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20 °C to -70 °C. Stock solutions can also be prepared at 50 - 100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2 - 1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in PBS.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Antigendetails

Target: Prolactin (PRL)

Andere Bezeichnung: Prolactin

Hintergrund: Prolactin, also known as luteotropin, is a hormone that is secreted by the pituitary gland and is best known for its role in enabling female mammals to produce milk. However, prolactin has also been shown to play essential roles in the regulation of immune functions, pancreatic development, angiogenesis, reproduction, osmoregulation, and metabolism. Besides the pituitary origin, prolactin is found in other tissues and cells such as adipocytes, immune cells, skin, prostate, brain, and decidua. This hormone is produced in increasing amounts during pregnancy and suckling. It acts on the mammary gland by initiating and maintaining lactation in the postpartum period. Epidemiological studies have demonstrated that circulating high levels of prolactin increase the risk of breast cancer. Prolactin may promote mammary tumor initiation via the JAK2/STAT5 signaling pathway. Human prolactin has a 60 % amino acid sequence homology with its murine counterpart. In a knockout animal study, it was shown that female mice lacking the expression of prolactin were infertile, but does not prevent them from manifesting spontaneous maternal behaviors. In addition, these animals show no sign of alterations in myelopoiesis and lymphopoiesis, suggesting that prolactin may not play an indispensable role in lymphocyte development and myeloid differentiation.

Molekulargewicht: The 200 amino acid recombinant protein has a predicted molecular mass of approximately 23 kDa. The protein migrates approximately at 25 kDa in DTT-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is Met.

Pathways: JAK-STAT Signalweg, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Protein targeting to Nucleus