APCS Protein (AA 20-223, C-Term)

Produktnummer ABIN2666528

Dieses Recombinant APCS-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für APCS Protein (AA 20-223, C-Term) (ABIN2666528)

Target: APCS (Amyloid P Component, Serum (APCS))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Reinheit: > 95 % , as determined by Coomassie stained SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 20-223, C-Term

Sterilität: 0.22 μm filtered

Endotoxin-Niveau:

Less than 1.0 EU per μg of protein as determined by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: Human APCS is able to bind immobilized HDL (50 μg/ml) in a dose dependent manner with a half maximum binding of ED50 = 0.24 - 0.96 μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: For maximum results, quick spin vial prior to opening.

Buffer: 0.22 μm filtered protein solution is in PBS, pH 7.2.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored between 2° and 8°C for one month, at -20°C for three months, or at -70°C for six months.

Antigendetails

Target: APCS (Amyloid P Component, Serum (APCS))

Andere Bezeichnung: APCS

Hintergrund: APCS (amyloid P component, serum), also known as pentraxin 2 (PTX2), is a member of the pentraxin family, which has a characteristic non-covalently linked pentameric organization. This protein is characterized by its calcium-dependent ligand binding and distinctive flattened β-jellyroll structure similar to legume lectins. Human APCS shares a 79 % amino acid homology with its mouse counterpart. APCS is secreted by hepatocytes as part of the acute phase protein synthesys in response to infections. Upon binding to its ligands, APCS is involved in the activation of classical complement cascade. In a mouse model, APCS was shown to downregulate acute inflammation accompanying experimental autoimmune encephalomyelitis by regulating T cell activity. Besides its role in regulating immune response, APCS is found to be a component of amyloid deposits, which has the ability of stabilizing amyloid fibrils and protecting them from proteolytic degradation. Based on these studies, it was postulated that APCS may play a role in Alzheimer's disease and other amyloidoses. Interestingly, APCS is also identified as a component of basement membrane proteins, and is able to bind laminin, collagen IV, P-selectin, and fibronectin in a Ca2+ dependent manner. In addition, APCS is known to specifically localize in human atherosclerotic lesions, which suggests that it may play a role in atherogenesis. Functional studies revealed that APCS binds to lipoproteins, specifically to HDL, but not LDL.

Molekulargewicht: The 219 amino acid recombinant protein has a predicted molecular mass of approximately 25 kDa. The protein migrates at 27 kDa in DTT-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is His.