CD14 Protein (CD14) (Fc Tag)

Produktnummer ABIN2566368

Dieses Recombinant CD14-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für CD14 Protein (CD14) (Fc Tag) (ABIN2566368)

Target: CD14

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Applikation: Western Blotting (WB)

Reinheit: >97 % as determined by SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses CD14 Protein ist gelabelt mit Fc Tag.

Produktmerkmale:

Measured by its ability to enhance LPS-stimulated IL-8 secretion by THP-1 human acute monocytic leukemia cells. The ED50 for this effect is typically 0. 5-4 μg/mL.

Fusion tag: C-Fc Tag

Anwendungsinformationen

Applikationshinweise: This recombinant protein can be used for WB. For research use only.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 50 mM tris, 100 mM glycine, pH 7.5

Lagerung: -80 °C,-20 °C

Informationen zur Lagerung: Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.

Antigendetails

Target: CD14

Andere Bezeichnung: CD14

Hintergrund: Cluster of differentiation 14 (CD14), is a cell surface glycoprotein, and is a is a component of the innate immune system. CD14 is a myelomonocytic differentiation antigen preferentially expressed on monocytes, macrophages, and activated granulocytes. CD14 exists in two forms. Either it is anchored into the membrane by a glycosylphosphatidylinositol tail (mCD14) or it appears in a soluble form (sCD14). Soluble CD14 either appears after shedding of mCD14 (48 kDa) or is directly secreted from intracellular vesicles (56 kDa). CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). CD14 has been proposed to be involved in various biological processes, including transportation of other lipids, cell-cell interaction during different immune responses, as well as recognition of apoptotic cells. Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns. CD14+ cells are monocytes that can differentiate into a host of different cells. CD14 has been shown to interact with Lipopolysaccharide-binding protein.

Molekulargewicht: 62.4 kDa

Gen-ID: 929

UniProt: P08571

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades