CD40 Ligand Protein (CD40LG) (Soluble)

Produktnummer ABIN2469083

Dieses Recombinant CD40 Ligand-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für CD40 Ligand Protein (CD40LG) (Soluble) (ABIN2469083)

Target: CD40 Ligand (CD40LG)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Reinheit: < 98 % by SDS-PAGE gel and HPLC analyses.

Produktdetails

Proteineigenschaft: Soluble

Sequenz: MQRGDEDPQI AAHVVSEANS NAASVLQWAK KGYYTMKSNL VMLENGKQLT VKREGLYYVY TQVTFCSNRE PSSQRPFIVG LWLKPSSGSE RILLKAANTH SSSQLCEQQS VHLGGVFELQ AGASVFVNVT EASQVIHRVG FSSFGLLKL

Produktmerkmale: Biological activity was determined by its ability to induce TNF - alpha and MIP - 1 alpha production by murine splenocytes. The expected ED50 for this effect is < 0.1 μg/mL.

Endotoxin-Niveau: Endotoxin level is less than 0.1 ng per μg (1 EU/μg).

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Handhabung: As with any protein, exposing Soluble CD40 Ligand recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Lagerung: -20 °C

Informationen zur Lagerung: The recombinant protein is stable for at least 2 years from date of receipt at -20 °C. Reconstituted Soluble CD40 Ligand is stable for at least 3 months when stored in working aliquots with a carrier protein at -20 °C.

Haltbarkeit: 24 months

Antigendetails

Target: CD40 Ligand (CD40LG)

Andere Bezeichnung: CD40 Ligand

Hintergrund: CD40, a member of the TNF receptor family, is a cell surface protein expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells, and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40-Ligand is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to "immunodeficiency with hyper-IgM", a disease characterized by failure to produce IgG, IgA and IgE. The soluble form of CD40L is an 18 kDa protein comprising the entire TNF homologous region of CD40L and is generated in vivo by an intracellular proteolytic processing of the full length CD40L. Recombinant murine CD40L is a soluble 16.4 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L.

Gen-ID: 21939

NCBI Accession: NP_035741

OMIM: 70608202

UniProt: P27512

Pathways: NF-kappaB Signalweg, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints