FASL Protein (Soluble)

Produktnummer ABIN2469071

Dieses Recombinant FASL-Protein wird in CHO Cells produziert.

Kurzübersicht für FASL Protein (Soluble) (ABIN2469071)

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: CHO Cells

Reinheit: <= 95 % by SDS-PAGE gel and HPLC analyses.

Produktdetails

Proteineigenschaft: Soluble

Sequenz: HHHHHHHHPS PPPEKKELRK VAHLTGKSNS RSMPLEWEDT YGIVLLSGVK YKKGGLVINE TGLYFVYSKV YFRGQSCNNL PLSHKVYMRN SKYPQDLVMM EGKMMSYCTT GQMWARSSYL GAVFNLTSAD HLYVNVSELS LVNFEESQTF FGLYKL

Produktmerkmale: Biological activity was determined by it's ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The ED50 for this effect is < 10.0 ng/mL, corresponding to a specific activity of > 1 x 10⁵ units/mg.

Endotoxin-Niveau: Endotoxin level is less than 0.1 ng per μg (1 EU/μg).

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Handhabung: As with any protein, exposing sFas Ligand recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Lagerung: -20 °C

Informationen zur Lagerung: The recombinant protein is stable for at least 2 years from date of receipt at -20 °C. Reconstituted sFas Ligand is stable for at least 3 months when stored in working aliquots with a carrier protein at -20 °C.

Haltbarkeit: 24 months

Antigendetails

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Andere Bezeichnung: Fas Ligand

Hintergrund: Fas Ligand (FasL) is a member of the TNF superfamily that is expressed on the cell surface of activated T cells. Binding of FasL to Fas Receptor triggers apoptosis in Fas-bearing cells. FasL has the ability to kill T cells and activated B cells which leads to down-regulation of the immune response. The mechanism of Fas induced apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. Both human and murine sFasL are fully active on human and murine cells. Recombinant human soluble Fas Ligand is a 17.9 kDa protein comprising the TNF homologous region of FasL and contains an 8 residue N-terminal His-Tag.

Gen-ID: 356

NCBI Accession: NP_000630

OMIM: 4557329

UniProt: P48023

Pathways: Apoptose, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity