Sonic Hedgehog Protein (SHH) (AA 24-197, Cys24IleIle-Mutant)

Produktnummer ABIN2018361

Recombinant Sonic Hedgehog-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für Sonic Hedgehog Protein (SHH) (AA 24-197, Cys24IleIle-Mutant) (ABIN2018361)

Target: Sonic Hedgehog (SHH)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: > 95 % by SDS-PAGE and HPLC analyses.

Produktdetails

Proteineigenschaft: AA 24-197, Cys24IleIle-Mutant

Produktmerkmale: ED50 < 2.0 μg/mL, measured by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) Cells, corresponding to a specific activity of > 500 units/mg.

Endotoxin-Niveau: < 0.2 EU/μg, determined by LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Lagerung: -80 °C

Informationen zur Lagerung: Lyophilized recombinant human Sonic Hedgehog (C24II) (rhShh) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhShh should be stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Haltbarkeit: 6 months

Antigendetails

Target: Sonic Hedgehog (SHH)

Hintergrund: Sonic Hedgehog (Shh) is a member of the Hedgehog (Hh) family of highly conserved proteins which are widely represented throughout the animal kingdom. In mammal, there are three related Hh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh). They share a high degree of amino-acid sequence identity (e.g., Shh and Ihh are 93 % identical). Sonic Hedgehog plays a role in cell growth, cell specialization, and the normal shaping (patterning) of the body. Shh is also important for development of the brain and spinal cord (central nervous system), eyes, limbs, and many other parts of the body.Recombinant human Sonic Hedgehog (C24II) (rhShh) produced in E. coli is a single non-glycosylated polypeptide chain containing 175 amino acids. A fully biologically active molecule, rhShh has a molecular mass of 19.7 kDa analyzed by reducing SDS-PAGE.
Synonyms: Hhg1

Molekulargewicht: 19.7 kDa, observed by reducing SDS-PAGE.

UniProt: Q15465

Pathways: Hedgehog Signalweg, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development