HGF Protein (AA 32-494, AA 495-728)

Produktnummer ABIN2017955

Produktdetails

Target: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 32-494, AA 495-728

Spezies: Human

Quelle: CHO Cells

Produktmerkmale: ED50 < 10 ng/mL, measured in a cell proliferation assay using 4MBr5 cells, corresponding to a specific activity of > 1x10^5 units/mg.
Alpha chain: AA 32-494, beta chain: AA 495-728

Reinheit: > 95 % as analyzed by SDS-PAGE.

Endotoxin-Niveau: < 0.2 EU/μg, determined by LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Lagerung: -80 °C

Informationen zur Lagerung: Lyophilized recombinant humanHepatocyte Growth Factor (HGF) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, human Hepatocyte Growth Factor (HGF) should be stable up to 1 week at 4 °C or up to 2 months at -20 °C.

Haltbarkeit: 6 months

Antigendetails

Target: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Andere Bezeichnung: Hepatocyte Growth Factor (HGF) (HGF Produkte)

Hintergrund: Hepatocyte Growth Factor (HGF),also known as hepatopoietin-A and scatter factor, is a pleiotropic mitogen belonging to the peptidase S1 family (plasminogen subfamily). It is produced by mesenchymal cells and acts on epithelial cells, endothelial cells and haemopoietic progenitor cells. HGF binds to the proto-oncogenic c-Met receptor to activate a tyrosine kinase signaling cascade. It regulates cell growth, motility and morphogenesis, thus it plays a pivotal role in angiogenesis, tumorogenesis and tissue regeneration.Recombinant human Hepatocyte Growth Factor (rhHGF) is produced in CHO cells and consists of two polypeptide chains (alpha-chain and beta-chain) held by a single disulfide bond resulting in the formation of a biologically active heterodimer. The alpha-chain consists of 463 amino acid residues and four kringle domains. The beta-chain consists of 234 amino acid residues. A fully biologically active molecule, rhHGF has a molecular mass of around 88-90 kDa analyzed by reducing SDS-PAGE.
Synonyms:

Molekulargewicht: 88-90 kDa (single chain), 59-61kDa (alpha chain), 30-34kDa (beta chain), observed by reducing SDS-PAGE.

Pathways: RTK Signalweg, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor