ATG5 Protein (AA 1-275)

Produktnummer ABIN1692441

Produktdetails

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-275

Spezies: Human

Quelle: Escherichia coli (E. coli)

Verwendungszweck: Recombinant Human Autophagy Related 5 Homolog/ATG5

Sequenz: MTDDKDVLRD VWFGRIPTCF TLYQDEITER EAEPYYLLLP RVSYLTLVTD KVKKHFQKVM RQEDISEIWF EYEGTPLKWH YPIGLLFDLL ASSSALPWNI TVHFKSFPEK DLLHCPSKDA IEAHFMSCMK EADALKHKSQ VINEMQKKDH KQLWMGLQND RFDQFWAINR KLMEYPAEEN GFRYIPFRIY QTTTERPFIQ KLFRPVAADG QLHTLGDLLK EVCPSAIDPE DGEKKNQVMI HGIEPMLETP LQWLSEHLSY PDNFLHISII PQPTD

Produktmerkmale:

Recombinant Human Autophagy Related 5 Homolog/ATG5

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM Tris,0.2M NaCl,1 mM DTT,40 % glycerol, pH 8.0 .

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Lagerung: -80 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Andere Bezeichnung: Autophagy Protein 5/ATG5 (ATG5 Produkte)

Synonyme: APG5 Protein, APG5-LIKE Protein, APG5L Protein, ASP Protein, hAPG5 Protein, ATG5 Protein, CG1643 Protein, DmAtg5 Protein, Dmel\\CG1643 Protein, atg5 Protein, 2010107M05Rik Protein, 3110067M24Rik Protein, AW319544 Protein, Apg5l Protein, Atg5l Protein, C88337 Protein, Paddy Protein, apg5l Protein, zgc:100934 Protein, ATATG5 Protein, AUTOPHAGY 5 Protein, MKP11.20 Protein, MKP11_20 Protein, autophagy related 5 Protein, Autophagy-related 5 Protein, ATG5 autophagy related 5 homolog (S. cerevisiae) Protein, autophagy related 5 L homeolog Protein, similar to S. cerevisiae ATG5 (YPL149W) which nucleates preautophagosome formation as a conjugate with Atg12 Protein, autophagy protein Apg5 family Protein, ATG5 Protein, Atg5 Protein, atg5 Protein, atg5.L Protein, APG5 Protein

Hintergrund: Recombinant Human Autophagy protein 5/ATG5 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Asp275) of Human ATG5.
ATG5 is an E2 ubiquitin ligase which is necessary for autophagy. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity, dramatically highly expressed in apoptotic cells. It is activated by ATG7, conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate complex detaches from the membrane immediately before or after autophagosome formation is completed. ATG5 plays an important role in the apoptotic process, possibly within the modified cytoskeleton.

Molekulargewicht: 32.4 kDa

UniProt: Q9H1Y0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagie