Leptin Receptor Protein (LEPR) (C-Term, Extracellular Domain)

Produktnummer ABIN1684639

Dieses Recombinant Leptin Receptor-Protein wird in HEK-293T Cells produziert.

Kurzübersicht für Leptin Receptor Protein (LEPR) (C-Term, Extracellular Domain) (ABIN1684639)

Target: Leptin Receptor (LEPR)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293T Cells

Reinheit: > 98 %, as determined by SDS-PAGE and HPLC

Produktdetails

Proteineigenschaft: C-Term, Extracellular Domain

Spezifität: Optimized DNA sequence encoding extracellular domain of human leptin receptor including a C-terminal Human IgG1 Fc tag was expressed in HEK293 cells.

Produktmerkmale: Recombinant human leptin receptor is a homodimer protein consisting of two 1070 amino acid residue subunit chains, due to glycosylation migrates as an approximately 160kDa protein on SDS-PAGE.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

Anwendungsinformationen

Kommentare:

Biologically active: Measured by its ability to bind immobilized recombinant human Leptin (1 µg/mL) at a linear concentration range of 0.01-4 µg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS solution, pH7.2

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: The lyophilized antibody is stable for at least 1 year from date of receipt at -20 °C. Upon reconstitution, this antibody can be stored in working aliquots at - 8 °C for one month, or at -20 °C for six months without detectable loss of activity.

Haltbarkeit: 12 months

Antigendetails

Target: Leptin Receptor (LEPR)

Andere Bezeichnung: LEPR

Hintergrund: FGF-10 is involved in the initial budding as well as the continuous outgrowth of vertebrate limbs, FGF10 mRNA is expressed preferentially in neurons but not in glial cells and may have a distinct role in the brain. Human FGF-10 is mitogenic for fetal rat keratinizing epidermal cells but not for NIH 3T3 cells. Recombinant FGF-10 induces the proliferation of human urothelial cells in vitro and induces the proliferation of transitional epithelium. FGF-10 is secreted by cultured mouse pre-adipocytes, prevention of FGF-10 signaling inhibits subsequent differentiation. The ability of embryonic fibroblasts derived from FGF-10 knock-out mice to differentiate into adipocytes is also impaired.

UniProt: P48357

Pathways: JAK-STAT Signalweg, AMPK Signaling, Feeding Behaviour