PLGF Protein (Homodimer)

Produktnummer ABIN1589565

Dieses Recombinant PLGF-Protein wird in Insektenzellen exprimiert.

Kurzübersicht für PLGF Protein (Homodimer) (ABIN1589565)

Target: PLGF (PGF) (Placenta Growth Factor (PGF))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Ratte

Quelle: Insektenzellen

Reinheit: > 95 % by SDS-PAGE

Produktdetails

Proteineigenschaft: Homodimer

Verwendungszweck: PlGF

Sequenz: ALSAGNNSTE MEVVPFNEVW GRSYCRPMEK LVYIADEHPN EVSHIFSPSC VLLSRCSGCC GDEGLHCVAL KTANITMQIL KIPPNRDPHS YVEMTFSQDV LCECRPILET TKAERRKTKG KRKQSKTPQT EEPHL

Spezifität: Chromosomal location:6q31

Produktmerkmale: Length (aa):135

Anwendungsinformationen

Applikationshinweise: Measured by its ability to bind to immobilized rh-sFlt-1 in a functional ELISA. Recombinant rat PlGF can bind to immobilized rh-sFlt-1 (100 ng/well) with a linear range at 0.1 - 5 ng/mL

Kommentare:

Cytokines & Growth Factors

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge vial prior to opening. The rat PlGF is supplied in lyophilized form and can be reconstituted with water. This solution can be diluted into other buffered solutions or stored frozen for future use.

Buffer: 25 mM Tris, pH 8.5

Lagerung: RT,-20 °C,-80 °C

Informationen zur Lagerung: The lyophilized rat PIGF, though stable at room temperature, is best stored in working aliquots at -20°C to -70°C.

Antigendetails

Target: PLGF (PGF) (Placenta Growth Factor (PGF))

Andere Bezeichnung: PlGF

Hintergrund: Placenta growth factor (PlGF) is a member of the PDGF/VEGF family of growth factors that share a conserved pattern of eight cysteines. Alternate splicing results in at least three human mature PlGF forms containing 131 (PlGF-1), 152 (PlGF-2), and 203 (PlGF-3) amino acids (aa) respectively. Only PlGF-2 contains a highly basic heparin-binding 21 aa insert at the C-terminus. In rat only one PlGF that is the equivalent of human PlGF-2 has been identified. Rat PlGF shares 60 %, 92 %, 62 % and 59% aa identity with the appropriate isoform of human, mouse, canine and equine PlGF. PlGF is mainly found as variably glycosylated, secreted, 55 - 60 kDa disulfide linked homodimers. Mammalian cells expressing PlGF include villous trophoblasts, decidual cells, erythroblasts, keratinocytes and some endothelial cells. Circulating PlGF increases during human pregnancy, reaching a peak in mid-gestation, this increase is attenuated in preeclampsia. However, deletion of PlGF in the mouse does not affect development or reproduction. Postnatally, mice lacking PlGF show impaired angiogenesis in response to ischemia. PlGF binds and signals through VEGF R1/Flt-1, but not VEGF R2/Flk-1/KDR, while VEGF binds both but signals only through the angiogenic receptor, VEGF R2. PlGF and VEGF therefore compete for binding to VEGF R1, allowing high PlGF to discourage VEGF/VEGF R1 binding and promote VEGF/VEGF R2-mediated angiogenesis. However, PlGF (especially human PlGF-1) and some forms of VEGF can form dimers that decrease the angiogenic effect of VEGF on VEGF R2. PlGF-2, like VEGF164/165, shows heparin-dependent binding of neuropilin (Npn)-1 and Npn-2 and can inhibit nerve growth cone collapse. PlGF induces monocyte activation, migration, and production of inflammatory cytokines and VEGF. These activities facilitate wound and bone fracture healing, but also contribute to inflammation in active sickle cell disease and atherosclerosis. Circulating PlGF often correlates with tumor stage and aggressiveness, and therapeutic PlGF antibodies are being investigated to inhibit tumor growth and angiogenesis.
Synonyms: Pgf, Plgf, placental growth factor

Molekulargewicht: 15.14 kDa

Gen-ID: 94203

NCBI Accession: NM_053595, NP_446047

UniProt: Q63434

Pathways: VEGFR1 Specific Signals