Flagellin Protein (FliC)
Kurzübersicht für Flagellin Protein (FliC) (ABIN1344457)
Target
Alle Flagellin (FliC) Proteine anzeigenProtein-Typ
Spezies
Quelle
Reinheit
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Verwendungszweck
- Flagellin (high purity)
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Spezifität
- Binds to human and mouse TLR5 (Toll-like receptor 5).
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Kreuzreaktivität
- Human, Maus
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Endotoxin-Niveau
- <0.001EU/μg purified protein (LAL test, Lonza).
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Biological Activity Comment
- Activation of TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, beta-defensin 2 and matrilysin (MMP-7). Maturation of dendritic cells in in vitro and in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous and intranasal routes in mice. TLR5 can be stimulated in vitro by Flagellin (high Purity) at a concentration of 5-50ng /ml and in vivo at a starting concentration of 0.1-1μg /mouse.
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Flagellin (FliC) (AA 2-498) protein (His tag)
FliC Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Flagellin (FliC) proteinFliC Spezies: Borrelia burgdorferi Wirt: Escherichia coli (E. coli) Recombinant ELISA, WB, SDS, CP
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- 0.1 mg/mL after reconstitution
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Konzentration
- 0.1 mg/mL
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Buffer
- Contains PBS.
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Handhabung
- After reconstitution, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 6 months after receipt when stored at -20°C.
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Haltbarkeit
- 6 months
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- Flagellin (FliC)
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Andere Bezeichnung
- Flagellin
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Hintergrund
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FliC
Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) and the intracellular protein NLRC4 (NLR family CARD domain-containing protein 4).
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Molekulargewicht
- ~54kDa (SDS-PAGE)
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UniProt
- P06179
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Pathways
- Inflammasome
Target
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