TRAIL Protein (AA 95-281, Extracellular Domain, Soluble) (DYKDDDDK Tag)

Produktnummer ABIN1344414

Produktdetails

Target: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: Soluble, Extracellular Domain, AA 95-281

Spezies: Human, Maus

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses TRAIL Protein ist gelabelt mit DYKDDDDK Tag.

Applikation: SDS-PAGE (SDS)

Verwendungszweck: TRAIL, Soluble (human) (rec.)

Kreuzreaktivität: Human, Maus

Produktmerkmale: The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a FLAG®-tag.

Reinheit: >95 % (SDS-PAGE)

Endotoxin-Niveau: <0.01EU/μg purified protein (LAL test).

Biological Activity Comment: Induces apoptosis of human Jurkat T cells in a concentration range of 5-50ng/ml in the presence of 1μg/ml TNF Ligands Enhancer (Prod. No. AG-35B-0001). In the absence of TNF Ligands Enhancer, TRAIL, Soluble (human) (recombinant) is poorly active.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 100 μL sterile water.

Konzentration: 0.1 mg/mL

Buffer: Contains PBS.

Handhabung: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Haltbarkeit: 6 months

Antigendetails

Target: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Andere Bezeichnung: TRAIL (TNFSF10 Produkte)

Hintergrund:

Apo-2L, TNFSF10, CD253

TNF-related apoptosis-inducing ligand (TRAIL, Apo2L,CD253, TNFSF10) is a type II transmembrane protein of about 34 kDa. Like most members of the tumor necrosis factor (TNF) superfamily of cytokines TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. Active TRAIL forms trimers and specifically binds to fi ve distinct receptors: TRAIL-R1 (DR4, Apo2,CD261, TNFRSF10A), TRAIL-R2 (DR5, KILLER, TRICK2A,TRICK2B, CD262, TNFRSF10B), TRAIL-R3 (DcR1,LIT, TRID, CD263, TNFRSF10C), TRAIL-R4 (DcR2,TRUNDD, CD264, TNFRSF10D), and osteoprotegerin (OPG, OCIF, TNFRSF11B). Trimerized TRAIL triggers apoptosis upon ligation of cell surface TRAIL-R1 and/or TRAIL-R2 by inducingthe formation of the so-called multiprotein death-inducing signaling complex (DISC).

Molekulargewicht: ~23kDa (SDS-PAGE)

UniProt: P50591

Pathways: Apoptose, Positive Regulation of Endopeptidase Activity