FASL Protein (AA 103-281, Soluble) (DYKDDDDK Tag)
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- Target Alle FASL Proteine anzeigen
- FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
- Proteineigenschaft
- AA 103-281, Soluble
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Spezies
- Human, Maus
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Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- Dieses FASL Protein ist gelabelt mit DYKDDDDK Tag.
- Applikation
- SDS-PAGE (SDS)
- Verwendungszweck
- FasL, Soluble (human) (rec.)
- Kreuzreaktivität
- Human, Maus
- Produktmerkmale
- The extracellular domain of human FasL (aa 103-281) is fused at the N-terminus to a FLAG®-tag.
- Reinheit
- >95 % (SDS-PAGE)
- Endotoxin-Niveau
- <0.05EU/μg purified protein (LAL test).
- Biological Activity Comment
- Induces apoptosis of human Jurkat T cells at a concentration of <1ng/ml in the presence of 0.1 to 1μg/ml TNF Ligands Enhancer (Prod. No. AG-35B-0001). In the absence of TNF Ligands Enhancer, FasL is working at 50-100 fold higher concentrations.
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- Discover our top product FASL Protein
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Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 1-281) protein (His tag)
custom-made FASL Spezies: Human Wirt: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 134-281) (Active) protein (His tag)FASL Spezies: Human Wirt: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 1-281) protein (GST tag)FASL Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AP, AA
Fas Ligand (TNF Superfamily, Member 6) (FASL) proteinFASL Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant > 90 % pure SDS
Fas Ligand (TNF Superfamily, Member 6) (FASL) protein (Fc Tag)FASL Spezies: Human Wirt: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Reconstitute with 100 μL sterile water.
- Konzentration
- 0.1 mg/mL
- Buffer
- Contains PBS.
- Handhabung
- After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
- Haltbarkeit
- 6 months
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- Target
- FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))
- Andere Bezeichnung
- FasL (FASL Produkte)
- Hintergrund
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APO-1L, CD95L, CD178, TNFSF6, Fas Ligand
FasL is a cytokine that binds to TNFRSF6/Fas, a receptor that transduces the apoptotic signal into cells. Is involved in cytotoxic T cell mediated apoptosis and in T cell development.
- Molekulargewicht
- ~33kDa (SDS-PAGE)
- UniProt
- P48023
- Pathways
- Apoptose, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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