LIF Protein (AA 23-202) (GST tag)

Produktnummer ABIN1344370

Produktdetails

Target: LIF (Leukemia Inhibitory Factor (LIF))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 23-202

Spezies: Human, Maus

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses LIF Protein ist gelabelt mit GST tag.

Applikation: SDS-PAGE (SDS)

Verwendungszweck: LIF (human) (rec.)

Spezifität: Mouse and human embryonic stem cells.

Kreuzreaktivität: Human, Maus

Produktmerkmale: Human LIF (aa 23-202) is fused at the N-terminus to a tag.

Reinheit: >95 % (SDS-PAGE)

Endotoxin-Niveau: <0.05EU/μg purified protein (LAL test).

Biological Activity Comment: Maintains pluripotency of mouse and human embryonic stem cells. The LIF (GST-tagged) from AdipoGen has the same activity as cleaved LIF.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: Lot specific

Buffer: Contains PBS.

Handhabung: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Haltbarkeit: 6 months

Antigendetails

Target: LIF (Leukemia Inhibitory Factor (LIF))

Andere Bezeichnung: LIF (LIF Produkte)

Hintergrund:

Leukemia Inhibitory Factor, Differentiation-stimulating Factor, D Factor, Melanoma-derived LPL Inhibitor

LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities also include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation and the stimulation of acute-phase protein synthesis in hepatocytes. LIF activates JAK & STAT signaling in human embryonic stem (ES) cells, but this pathway does not maintain pluripotency in these cells, which instead rely on FGF2-mediated ERK signaling. By contrast, mouse ES cells can be maintained by LIF-mediated JAK & STAT signaling. LIF binds to a high affinity heterodimeric receptor complex consisting of two proteins: LIF-R alpha that binds LIF with low affinity and the 130 kDa (gp130) subunit that by itself does not bind LIF, but is required for high affinity binding of LIF.

Molekulargewicht: ~46kDa (SDS-PAGE)

UniProt: P15018

Pathways: JAK-STAT Signalweg, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding