HMGB1 Protein (BoxB)

Produktnummer ABIN1176819

Recombinant HMGB1-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für HMGB1 Protein (BoxB) (ABIN1176819)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human, Maus

Quelle: Escherichia coli (E. coli)

Applikation: Activation (Act), Cellular Assay (CA)

Reinheit: > 95 % It contains no nucleic acids.

Produktdetails

Proteineigenschaft: BoxB

Verwendungszweck: BoxB from HMGB1, human & mouse, LPS-Free

Sequenz: Box B from HMGB1 consists of 90 aminoacid residues: MARIDPNAPK RPPSAFFLFC SEYRPKIKGE HPGLSIGDVA KKLGEMWNNT AADDKQPYEK KAAKLKEKYE KDIAAYRAKG KPDAAKKGVV

Produktmerkmale: BoxB recapitulates the cytokine activity of full length HMGB1 and efficiently activates macrophages to release tumor necrosis factor (TNF- ) and other proinflammatory cytokines (Li et al 2004).
BoxB from HMGB1 is produced in E.coli from an expression plasmid coding for the mammalian sequence, which is totally identical in human and mouse, preceded by four amino acids (MARI) that have been added for the purpose of expression in bacteria.

Aufreinigung: Purified. Oxidation of cysteine 106 makes the protein inactive. To avoid cysteine oxidation DTT 0.5 mM is added during protein purification.

Endotoxin-Niveau: LPS free: Pierce™ Chromogenic Endotoxin Quant Kit, <0.1 EU/mL

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: The lyophilized protein once reconstituted with distilled water will be dissolved in a solution containing 50 mM HEPES pH 7.9, 500 mM NaCl, 0.5mM DTT.

Buffer: The protein is lyophilized from 50 mM HEPES buffer pH 7.9, 500 mM NaCl, 0.5 mM DTT.
The product contains contains < 0.006% v/v of Triton X-114 due to LPS removal. Remaining traces can be removed upon request.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: 2-8°C when lyophilized. The protein once reconstituted with water can be stored frozen (-20°C).

Antigendetails

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Andere Bezeichnung: High-mobility group protein B1 (HMGB1)

Hintergrund: HMGB1 is a nuclear protein that is released passively by necrotic cells, retained by apoptotic cells, and secreted actively by inflammatory cells.

Molekulargewicht: 10 kDa

Pathways: p53 Signalweg, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome