HMGB1 Protein (BoxA Domain)

Produktnummer ABIN1176814

Recombinant HMGB1-Protein exprimiert in Escherichia coli (E. coli) und wurde in 1 Publikation erwähnt.

Kurzübersicht für HMGB1 Protein (BoxA Domain) (ABIN1176814)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human, Maus

Quelle: Escherichia coli (E. coli)

Applikation: Activation (Act), Cellular Assay (CA)

Reinheit: > 98 %

Produktdetails

Proteineigenschaft: BoxA Domain

Sequenz: MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKF

Produktmerkmale: BoxA is an antagonist for HMGB1 and appears to inhibit all its activities, depending from all receptors. Injection of 600 µg BoxA in the mouse protects against sepsis in a peritonitis model and from hepatitis in a mouse model of HBV infection (Sitia et al 2007). BoxA (10 µg/mL) inhibits maturation, survival and Th1 differentiation of dendritic cells and T cell proliferation.It contains only trace amounts of LPS (<0.4 ng/mg protein), and it is tested for the ability to inhibit the chemotactic activity of HMGB1 on fibroblasts.

Aufreinigung: Purified

Endotoxin-Niveau: The purified protein is free from LPS (Cambrex Limulus Amoebocyte Assay QCL-1000, <0.4 ng LPS per mg protein).

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 50 mM HEPES-Na pH 7.9, 500 mM NaCl, 0.5 mM DTT.

Handhabung: The product is resistant to repeated freezing and thawing.

Lagerung: -20 °C

Referenzen

Maroso, Balosso, Ravizza, Liu, Aronica, Iyer, Rossetti, Molteni, Casalgrandi, Manfredi, Bianchi, Vezzani: "Toll-like receptor 4 and high-mobility group box-1 are involved in ictogenesis and can be targeted to reduce seizures." in: Nature medicine, Vol. 16, Issue 4, pp. 413-9, (2010) (PubMed).

Antigendetails

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Andere Bezeichnung: High-mobility group protein B1 (HMGB1)

Hintergrund: BoxA is one of the highly conserved DNA binding domains of HMGB1 protein. It consists of 89 amino acids and has a calculated molecular mass of approximately 10.4 kDa. The sequence of BoxA is totally identical in human and mouse.

Molekulargewicht: approximately 10.4 kDa

Pathways: p53 Signalweg, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome