HMGB1 Protein (BoxA)

Produktnummer ABIN1176814

Recombinant HMGB1-Protein exprimiert in Escherichia coli (E. coli) und wurde in 1 Publikation erwähnt.

Kurzübersicht für HMGB1 Protein (BoxA) (ABIN1176814)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human, Maus

Quelle: Escherichia coli (E. coli)

Applikation: Activation (Act), Cellular Assay (CA)

Reinheit: > 98 % It contains no nucleic acids.

Produktdetails

Proteineigenschaft: BoxA

Verwendungszweck: BoxA from HMGB1, human & mouse, LPS-Free

Sequenz: BoxA from HMGB1 consists of 89 amino acid: MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKF

Spezifität: BoxA is one of the highly conserved DNA binding domains of HMGB1 protein (HMG boxes), and inhibits HMGB1 effects in vivo.

Produktmerkmale: The BoxA we provide has no difference from the sequence of the human or mouse HMGB1, and as a self-antigen elicits very low immunological responses.
BoxA protects against sepsis in a mouse peritonitis model (Yang et al. 2004), and from hepatitis in a mouse model of HBV infection (Sitia et al. 2007).
Dendritic cells promote their own maturation and T cell proliferation, survival and Th1 differentiation by secreting HMGB1, and BoxA inhibits these activities (Dumitriu et al. 2005).
BoxA also inhibits the chemotactic activity of HMGB1 and the mitogenic activity of HMGB1 on stem cells.
BoxA from HMGB1 is produced in E.coli from an expression plasmid coding for the unmodified mammalian sequence, which is totally identical in human and mouse.

Aufreinigung: Purified. Oxidation of cysteine 106 makes the protein inactive. To avoid cysteine oxidation DTT 0.5 mM is added during protein purification.

Endotoxin-Niveau: LPS free: Pierce™ Chromogenic Endotoxin Quant Kit, <0.1 EU/mL

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: The lyophilized protein once reconstituted with distilled water will be dissolved in a solution containing 50 mM HEPES pH 7.9, 500 mM NaCl, 0.5 mM DTT.

Buffer: The protein is lyophilized from 50 mM HEPES-Na pH 7.9, 500 mM NaCl, 0.5 mM DTT.
The product contains contains < 0.006% v/v of Triton X-114 due to LPS removal. Remaining traces can be removed upon request.

Handhabung: The product is resistant to repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: 2-8°C when lyophilized. The protein once reconstituted with water can be stored frozen (-20°C). The product is resistant to repeated freezing and thawing.

Referenzen

Maroso, Balosso, Ravizza, Liu, Aronica, Iyer, Rossetti, Molteni, Casalgrandi, Manfredi, Bianchi, Vezzani: "Toll-like receptor 4 and high-mobility group box-1 are involved in ictogenesis and can be targeted to reduce seizures." in: Nature medicine, Vol. 16, Issue 4, pp. 413-9, (2010) (PubMed).

Antigendetails

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Andere Bezeichnung: High-mobility group protein B1 (HMGB1)

Hintergrund: HMGB1 is a nuclear protein that is released passively by necrotic cells, retained by apoptotic cells, and secreted actively by inflammatory cells.

Molekulargewicht: 10.4 kDa

Pathways: p53 Signalweg, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome