APEX1 Protein (AA 2-318)

Produktnummer ABIN1096032

Produktdetails

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-318

Spezies: Human

Quelle: Escherichia coli (E. coli)

Verwendungszweck: Recombinant Human Apurinic-Apyrimidinic Endonuclease 1/APE

Sequenz: MGPKRGKKGA VAEDGDELRT EPEAKKSKTA AKKNDKEAAG EGPALYEDPP DQKTSPSGKP ATLKICSWNV DGLRAWIKKK GLDWVKEEAP DILCLQETKC SENKLPAELQ ELPGLSHQYW SAPSDKEGYS GVGLLSRQCP LKVSYGIGEE EHDQEGRVIV AEFDSFVLVT AYVPNAGRGL VRLEYRQRWD EAFRKFLKGL ASRKPLVLCG DLNVAHEEID LRNPKGNKKN AGFTPQERQG FGELLQAVPL ADSFRHLYPN TPYAYTFWTY MMNARSKNVG WRLDYFLLSH SLLPALCDSK IRSKALGSDH CPITLYLAL

Produktmerkmale: Recombinant Human Apurinic-Apyrimidinic Endonuclease 1/APE1 is produced with our E. coli expression system. The target protein is expressed with sequence (Pro2-Leu318) of Human APE1.

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 10 mM HEPES, 100 mM KCl, 50 % Glycerol, pH 7.4.

Handhabung: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Lagerung: -80 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Andere Bezeichnung: APE1 (APEX1 Produkte)

Hintergrund: Apurinic-Apyrimidinic Endonuclease 1 (APE1) is required for efficient DNA base excision repair. When the DNA glycosylase remove the damaged bases, APE1 cleaves the AP site to allow resynthesis and ligation to complete repair. APE1 stimulates the DNA binding activity of many transcription factors, which participate in cancer promotion and progression. APE1 regulates the redox state of multiple transcription factors, such as c-Jun, c-Fos, NF-kB, p53. APEN is also involved in calcium-dependent down-regulation of PTH expression.
Alternative Names: DNA-(Apurinic or Apyrimidinic Site) Lyase, APEX Nuclease, APEN, Apurinic-Apyrimidinic Endonuclease 1, AP Endonuclease 1, APE-1 REF-1, Redox Factor-1, APEX1, APE, APE1, APEX, APX, HAP1, REF1

Molekulargewicht: 35.62 kDa

UniProt: P27695

Pathways: DNA Reparatur, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus