UNG Protein

Produktnummer ABIN1068292

Dieses UNG-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für UNG Protein (ABIN1068292)

Target: UNG (Uracil-DNA Glycosylase (UNG))

Biologische Aktivität: Active

Spezies: E. coli

Quelle: Escherichia coli (E. coli)

Produktdetails

Produktmerkmale: 10x UNG Reaction Buffer: 200mM Tris-HCl (pH 8.0 at 25°C), 10mM DTT and 10mM EDTA.

Sterilität: Sterile filtered

Unit Definition: 1 Unit of the enzyme catalyzes the release of 1 nanomole of uracil-containing DNA template in 60 min at 37°C.

Anwendungsinformationen

Kommentare:

Specific Activity: The Specific Activity was found to be 5U/μl.
Reaction Conditions: 1X UNG Reaction Buffer, incubate at 37°C.UNG is active over a broad pH rabge with an optimum at pH-8.0, doesn't require divalent cation, and is inhibited by high ionic strength (>200mM). The abasic sites formed in DNA by UNG may be cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites.
Inhibition and Inactivation: Inactivated by heating at 95°C for 10min. Enzye activity is partially restored at temperatures lower than 55°C.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: UNG solution(5U/ul)in 10mM Tris-Hcl (pH-7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.1 mg/ml BSA and 50% glycerol.

Informationen zur Lagerung: Uracil DNA Glycosilase although stable at 15°C for 1 week, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.

Antigendetails

Target: UNG (Uracil-DNA Glycosylase (UNG))

Andere Bezeichnung: Uracil DNA Glycosylase

Substanzklasse: Viral Protein

Hintergrund: E.Coli Uracil DNA Glycosilase (UNG) catalyses the release of free Uracil from Uracil-containing DNA. UNG efficiently hydrolyzes uracil from signle-stranded or double-stranded DNA, but not from oligomers (6 fewer bases).

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response