VEGFA ELISA Kit
Kurzübersicht für VEGFA ELISA Kit (ABIN8126240)
Target
Alle VEGFA ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Detektionsbereich
Applikation
Proben
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Untere Nachweisgrenze
- 31.2 pg/mL
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Verwendungszweck
- This assay employs an antibody specific for Anti-human VEGF coated on a 96-well plate.
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Analytische Methode
- Quantitative
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Sensitivität
- 9.0 pg/mL
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Produktmerkmale
- This immunoassay kit allows for the in vitro quantitative determination of Human VEGF concentrations in serum, plasma. Detection Range: 31.2 - 2000 pg/mL Sensitivity: < 9.0 pg/mL
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Plattentyp
- Pre-coated
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Protokoll
- This assay employs an antibody specific for Anti-human VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human VEGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Informationen zur Lagerung
- Please refer to the Manual
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- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
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Andere Bezeichnung
- VEGF (Vascular Endothelial Cell Growth Factor)
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Pathways
- RTK Signalweg, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
Target Alle VEGFA ELISA Kits anzeigen
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